Kidney Week

Abstract: TH-PO605

Changes in Gene Expression of Hypothalamic Neuropeptides Controlling Feeding Regulation in Bilaterally Nephrectomized Rats

Session Information

• Nutrition and Metabolism: Basic
  October 25, 2018 | Location: Exhibit Hall, San Diego Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Health Maintenance, Nutrition, and Metabolism

• 1301 Health Maintenance, Nutrition, and Metabolism: Basic

Authors

• Ueno, Hiromichi, University of Occupational and Environmental Health, Kitakyushu, Japan

Hiromichi Ueno,

• Miyamoto, Tetsu, Second Department of Internal Medicine, Kitakyushu, Fukuoka, Japan

Tetsu Miyamoto,

• Sanada, Kenya, University of Occupational and Environmental Health, Japan, Kitakyushu-shi, Japan

Kenya Sanada,

• Fujimoto, Yoko, University of Occupational and Environmental Health, Kitakyushu, Japan

Yoko Fujimoto,

• Bando, Kenichiro, University of occupational and environmental health, Kitakyushu, Japan

Kenichiro Bando,

• Ueta, Yoichi, University of Occupational and Environmental Health, Kitakyushu, Japan

Yoichi Ueta,

• Otsuji, Yutaka, University of Occupational and Environmental Health, Kitakyushu, Japan

Yutaka Otsuji,

• Tamura, Masahito, University of Occupational & Environmental Health, Kitakyushu, FUKUOKA, Japan

Masahito Tamura,

Background

Anorexia is one of the most widespread eating disorders that appears to contribute to malnutrition in patients with advanced renal dysfunction. While several mechanisms underlying uremic anorexia have been proposed, the hypothalamic neuropeptides that regulate feeding in the hypothalamus of uremic patients are poorly understood. These neuropeptides act both on the hypothalamus and other appetite-regulating centers throughout the brain.

Methods

We evaluated the gene expression of hypothalamic feeding-regulating neuropeptides after bilateral nephrectomy, a model of acute renal dysfunction. Adult male rats received bilateral nephrectomy or a sham operation. The rats were decapitated at 6, 12, and 24 h after treatment. The brains were removed immediately, frozen on dry ice. The brains were then cut into 12-μm-thick sections using a cryostat and the locations of the hypothalamic areas, including the paraventricular nucleus (PVN), arcuate nucleus, and lateral hypothalamic area, were determined according to the coordinates of a mouse brain atlas. The gene expression of corticotrophin-releasing hormone (CRH) in the PVN; propiomelanocortin (POMC), cocaine- and amphetamine-regulated transcript, neuropeptide Y, agouti-related peptide in the ARC; and melanin-concentrating hormone and orexin in the LHA, were quantified by in situ hybridization histochemistry. After treatment, cumulative food intake, water intake, and body weight were measured.

Results

The mRNA levels of POMC and CRH, which suppress feeding behavior, were significantly increased after bilateral nephrectomy compared with sham-operated rats. The food intake of nephrectomized rats decreased compared with sham-operated rats, but there was no difference in body weight and water intake between both groups.

Conclusion

The results suggest that POMC and CRH in the hypothalamus may be involved in the development of anorexia in bilateral nephrectomized rats. This report may provide new insights into the physiological mechanism underlying anorexia in patients with renal dysfunction.