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Abstract: FR-PO694

miRNAs and Hyalinizing Vasculopathy in Peritoneal Dialysis Patients

Session Information

Category: Dialysis

  • 703 Dialysis: Peritoneal Dialysis

Authors

  • Selgas, Rafael, Hospital University La Paz, Madrid, Spain
  • Rodrigues díez, Raúl R., Universidad Autonoma, Madrid, Spain
  • Ramos-Ruiz, Ricardo, parque científico de madrid, MADRID, Spain
  • Del peso, Gloria, Hospital Universitario La Paz, Madrid, Spain
  • Bajo, Maria A., Hospital University La Paz, Madrid, Spain
  • Ruiz-Ortega, Marta, Universidad Autonoma, Madrid, Spain
  • Lopez-Cabrera, Manuel, Consejo Superior de Investigaciones Cientificasn, Madrid, Spain
  • Llorens, Carlos, Biotechvana, Paterna, Spain

Group or Team Name

  • Peritoneal Studies Group in Madrid
Background

Peritoneal membrane is conditioned by lesions appearing at long-term for dialysis purposes. Among these lesions, Hialynazing Vasculopathy has received less attention than others, and mechanisms causing this damage are incompletely studied although it is regularly found at advanced peritoneal stages. miRNAs are important regulators of cell biology. A miRNA transcriptomic study is an appropriate approach to independently (non-hypothesis driven) explore molecular mechanisms involved in this pathology.

Methods

We studied 7 patients affected by peritoneal vasculopathy and 4 patients without it.
Total RNA was extracted from FFPE peritoneal tissue, small RNA libraries were prepared and sequenced.

Results

20 miRNAs differentially expressed between groups were found (Table, up/down in vasculopathy). Remarkably, 9 of them belong to a cluster of miRNA loci separated by less than 10 kb in chromosome 14 (marked in Table).
We also detected messenger RNAs which were differentially expressed, and positive correlations between miRNAs and these messenger RNAs were found

Conclusion

GO enrichment performed based on coding genes shown as differentially expressed, revealed differential enrichment in proteins with hydrolase activity and proteins with RNA and protein binding functions. Differential enrichment was also observed in proteins involved in translation processes with sub-localizations mainly exosomal vesicles and extracellular space besides of cytosol sub-localisations probably related with translational processes.Enrichment analysis of metabolic pathways reveals enrichment in enzymes involved in three pathways; Aminoacyl-tRNA biosynthesis, N-Glycan biosynthesis and Galactose metabolism.
The impact of these new findings in terms of gene function will be discussed.

Funding

  • Government Support - Non-U.S.