ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005


The Latest on Twitter

Kidney Week

Abstract: FR-PO473

Fludrocortisone Stimulates Erythropoietin (Epo) Protein Expression in the Distal Tubules of Mouse Kidney

Session Information

Category: Anemia and Iron Metabolism

  • 201 Anemia and Iron Metabolism: Basic


  • Yasuoka, Yukiko, Kitasato University, School of Medicine, Sagamihara, Kanagawa, Japan
  • Oshima, Tomomi, Kitasato University, School of Medicine, Sagamihara, Kanagawa, Japan
  • Izumi, Yuichiro, Kumamoto University, Kumamoto, Japan
  • Sato, Yuichi, Kitasato University, School of Allied Health Sciences, Sagamihara, Japan
  • Takahashi, Noriko, Kitasato University, School of Medicine, Sagamihara, Kanagawa, Japan
  • Nonoguchi, Hiroshi, Kitasato University Medical Center, Kitamoto, Saitama, Japan
  • Kawahara, Katsumasa, Kitasato University, School of Medicine, Sagamihara, Kanagawa, Japan

Although normal plasma Epo concentration is very low, Epo mRNA was expressed in the kidney tubules under normal condition (Nagai, Yasuoka, et al, 2014). We further showed that fludrocortisone, an aldosterone receptor agonist, stimulated Epo mRNA expression in the distal nephron (Yasuoka, et al, ASN 2017). In this study, we investigated the localization of Epo protein-producing cells in the kidney and liver after fludrocortisone injection.


Fludrocortisone (2.5 mg/100 g BW) or Angiotensin II (0.5 µg/100 g BW) was once applied to mice (C57BL/6J, male, 10 weeks). After 2, 4, 6 and 72 hr, kidneys were examined by Western blot and immunohistochemistry using anti-Epo antibodies.


Under normal (basal) conditions, Epo staining was widely found along the nephron (proximal tubules < thick ascending limbs < collecting ducts). Fludrocortisone significantly increased Epo production in the medullary thick ascending limb, and cortical and outer medullary collecting ducts, particularly in type A of intercalated cells after 4-6 hr. Western blot showed that Epo protein expression was increased by 5-fold in the kidney but not changed in the liver. Angiotensin II stimulated Epo mRNA expression in proximal and distal tubules but not in the interstitial cells. Epo staining decreased to the basal level after 72 hr, but was not detected in the interstitial cells.


Renin-angiotensin-aldosterone system regulates Epo production by the nephron.


  • Government Support - Non-U.S.