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Abstract: FR-PO080

Efficacy of Low Intensity Pulsed Ultrasound on an AKI Mouse Model via the Prevention of Endoplasmic Reticulum Stress and Apoptosis

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Liu, Shing-Hwa, National Taiwan University, Medical College, Taipei, taiwan, Taiwan
  • Hung, Kuan-Yu, National Taiwan University Hospital, TAIPEI, Taiwan
  • Chiang, Chih-Kang, National Taiwan University, Medical College, Taipei, Taiwan
Background

Acute kidney injury (AKI) is associated with high mortality rates and often predisposes patient to development of chronic kidney disease. Renal ischemia/reperfusion injury (IRI) is a major cause of AKI. The mechanisms of IRI have been found to be included endoplasmic reticulum (ER) stress, inflammatory responses, hypoxia, and generation of reactive oxygen species. Low intensity pulsed ultrasound (LIPUS), which is a kind of therapeutic ultrasound, has been shown to exert therapeutic effects on bone healing and accelerate the healing process. The effect and mechanism of LIPUS on AKI still remain unclear. Here, we investigated the therapeutic effect and possible mechanism of LIPUS on IRI.

Methods

We use a mouse model of unilateral IRI with nephrectomy of the contralateral kidney in the presence or absence of LIPUS treatment (3 MHz, intensity 0.1 W/cm2, 20 mins, 50% duty factor) 5 day before and 1 day after surgery.

Results

The results showed that renal function markers (e.g. BUN, creatinine), ER stress-related molecules (e.g. GRP78, eIF2-α, CHOP) and apoptotic markers (e.g. Bax, caspase-3) were significantly increased in the kidneys of mice with IRI for 24 h, which could be significantly reversed by LIPUS treatment. The histopathological examination showed that the lesser renal tubular injury and inflammation were observed in IRI+LIPUS mice than in IRI alone mice.

Conclusion

Taken together, LIPUS treatment showed the benefits for renal protection in IRI mice. These findings suggest that LIPUS therapy may be used to serve as an auxiliary tool for management of AKI.