Abstract: FR-PO561
Exosomal MicroRNAs Profiling in Obese Patient Using MicroRNA-Sequencing
Session Information
- Physical Activity, Body Composition, Metabolism: Clinical
October 26, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1302 Health Maintenance, Nutrition, and Metabolism: Clinical
Authors
- Kwon, Soon hyo, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
- Kim, Hyoungnae, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
- Park, Moo Yong, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
- Noh, Hyunjin, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
Background
Exosomal micro RNAs (miR) are valuable biomarker and regulate biological process of obesity. Bariatric surgery has beneficial effect on obese patients. However the mechanisms of this surgery are not clear. We investigated to identify exosomal miR signature associated with obesity and examine exosomal miR changes after bariatric surgery.
Methods
Healthy volunteers (HV, n=18) and non-diabetic obese patients (n=16) were enrolled prospectively. We assessed the miR profile of serum exosome from HV and obese patients using RNA sequencing (RNA-seq) cross sectionally. To evaluate the effect of bariatric surgery, we conducted exosomal miR analysis in 12 obese patients 6 months after surgery.
Results
RNA-seq analysis showed 224 obesity-related microRNAs. Using >2 and <-2-fold changes as cut-offs, we identified that the expression of 72 (up-regulation=57, down-regulation=15) exosomal miR in obese patients compared to HV (table 1). Biological pathway analysis was suggestive of modulation of signaling pathways including the Wnt pathway, fatty acid biosynthesis, AMPK signaling, p38 MAPK signaling, and AMPK signaling in exosome from obesity patients. Bariatric surgery induced weight reduction led to a marked change in the Wnt pathway and fatty acid biosynthesis pathway.
Conclusion
Obesity leads a different miR pattern in serum exosome compared to healthy volunteers. Weight loss after bariatric also induces changes in exosomal miR profiles.
Top 5 list of exosomal micro RNAs from obesity compared to healthy volunteers
| mature micro RNA | obesity/healthy volunteers fraction |
| hsa-miR-122-5p | 11.8 |
| hsa-miR-193b-5p | 6.8 |
| hsa-miR-4449 | 6.6 |
| hsa-let-7a-3p | 6.5 |
| hsa-miR-1290 | 5.9 |
| hsa-miR-26b-3p | 5.9 |
| hsa-let-7f-1-3p | 5.9 |
| hsa-miR-4461 | -14.3 |
| hsa-miR-6739-5p | -13.0 |
| hsa-miR-1273a | -10.5 |
Funding
- Government Support - Non-U.S.