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Abstract: FR-PO561

Exosomal MicroRNAs Profiling in Obese Patient Using MicroRNA-Sequencing

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1302 Health Maintenance, Nutrition, and Metabolism: Clinical

Authors

  • Kwon, Soon hyo, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
  • Kim, Hyoungnae, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
  • Park, Moo Yong, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
  • Noh, Hyunjin, Soonchunhyang University Hospital, Seoul, Korea (the Republic of)
Background

Exosomal micro RNAs (miR) are valuable biomarker and regulate biological process of obesity. Bariatric surgery has beneficial effect on obese patients. However the mechanisms of this surgery are not clear. We investigated to identify exosomal miR signature associated with obesity and examine exosomal miR changes after bariatric surgery.

Methods

Healthy volunteers (HV, n=18) and non-diabetic obese patients (n=16) were enrolled prospectively. We assessed the miR profile of serum exosome from HV and obese patients using RNA sequencing (RNA-seq) cross sectionally. To evaluate the effect of bariatric surgery, we conducted exosomal miR analysis in 12 obese patients 6 months after surgery.

Results

RNA-seq analysis showed 224 obesity-related microRNAs. Using >2 and <-2-fold changes as cut-offs, we identified that the expression of 72 (up-regulation=57, down-regulation=15) exosomal miR in obese patients compared to HV (table 1). Biological pathway analysis was suggestive of modulation of signaling pathways including the Wnt pathway, fatty acid biosynthesis, AMPK signaling, p38 MAPK signaling, and AMPK signaling in exosome from obesity patients. Bariatric surgery induced weight reduction led to a marked change in the Wnt pathway and fatty acid biosynthesis pathway.

Conclusion

Obesity leads a different miR pattern in serum exosome compared to healthy volunteers. Weight loss after bariatric also induces changes in exosomal miR profiles.

Top 5 list of exosomal micro RNAs from obesity compared to healthy volunteers
mature micro RNAobesity/healthy volunteers fraction
hsa-miR-122-5p11.8
hsa-miR-193b-5p6.8
hsa-miR-44496.6
hsa-let-7a-3p6.5
hsa-miR-12905.9
hsa-miR-26b-3p5.9
hsa-let-7f-1-3p5.9
hsa-miR-4461-14.3
hsa-miR-6739-5p-13.0
hsa-miR-1273a-10.5

Funding

  • Government Support - Non-U.S.