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Abstract: FR-PO544

Effect of Acute and Chronic Intradialytic Bicycle Ergometer Exercise on Nrf2 mRNA Expression in Hemodialysis Patients

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1302 Health Maintenance, Nutrition, and Metabolism: Clinical

Authors

  • Mafra, Denise, Federal University Fluminense, Rio de Janeiro, Rio De Janeiro, Brazil
  • Brito, Jessyca Sousa de, Universidade Federal Fluminense, RIO DE JANEIRO, RIO DE JANEIRO, Brazil
  • Da silva, Greicielle Santos, Universidade Federal Fluminense, RIO DE JANEIRO, RIO DE JANEIRO, Brazil
  • Reis, Drielly V., Universidade Federal Fluminense, RIO DE JANEIRO, RIO DE JANEIRO, Brazil
  • Souza, Bruna kelly Santos, Universidade Federal Fluminense, RIO DE JANEIRO, Brazil
  • Cardozo, Ludmila Fmf, Federal Fluminense University, Niterói, RIO DE JANEIRO, Brazil
  • Borges, Natalia Alvarenga, Universidade Federal Fluminense, RIO DE JANEIRO, RIO DE JANEIRO, Brazil
Background

Hemodialysis (HD) patients have a constant state of oxidative stress, which results from an imbalance between reactive oxygen species production and endogenous antioxidant defense mechanisms, leading to increased cardiovascular risk. Physical exercise may reduce cardiovascular risk through its beneficial effects on nuclear factor erythroid 2-related factor 2 (Nrf2) expression that regulates several antioxidant genes. The aim of the present study was to verify the effect of acute and chronic intradialytic bicycle ergometer exercise on Nrf2 mRNA expression in HD patients.

Methods

Eleven HD patients were studied (7 men, 47.5 ± 6.7 yrs, 25.4 ± 12.3 months of dialysis, BMI of 23.7 ± 2.2 Kg/m2). The physical exercise program was individualized and performed on an adapted stationary bicycle, consisting in heating (5 minutes), 35 minutes of aerobic training at target heart rate, and cool down (load reduction for 5 minutes). The acute effect (single session) was assessed after the first exercise session and the chronic effect after 3 months of the exercise program with 3 sessions per week. Peripheral blood mononuclear cells were isolated and processed for the evaluation of expression of Nrf2 and NAD(P)H:quinoneoxidoreductase 1 (NQO1) by quantitative real-time polymerase chain reaction.

Results

No significant changes were observed after a single exercise session in Nrf2 mRNA expression [from 0.38 (0.15 - 2.84) to 1.92 (0.65 - 2.48), p=0.21] or NQO1 mRNA expression [from 0.31 (0.16 -1.20) to 0.85 (0.43 - 1.98), p=0.17]. After 3 months of the exercise program there was a significant increase in Nrf2 mRNA expression [from 0.38 (0.15 -2.84) to 1.63 (0.64 - 3.25), p=0.04] and no significant change in NQO1 mRNA expression [from 0.31(0.16 -1.20) to 1.97 (0.45 - 3.88), p=0.17].

Conclusion

These results suggest that acute intradialytic bicycle ergometer exercise was unable to change Nrf2 but three months exercise program seems to modulate this transcription factor which could improve long-term endogenous antioxidant defense in HD patients.

Funding

  • Government Support - Non-U.S.