Abstract: SA-PO574
Fibroblast mTORC2/PPARγ-Dependent HGF Production Protects Against Tubular Cell Death and AKI
Session Information
- AKI: Other Mechanisms and Cell Cultures
October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Yuan, Gui, Nanjing medical university, Nanjing, China
- Dai, Chunsun, Nanjing medical university, Nanjing, China
Background
Kidney interstitial fibroblasts play a crucial role in dictating tubular cell fate and the outcome of acute kidney injury (AKI). However, the underlying mechanisms remain to be further determined.
Methods
Mouse model with inducible Rictor gene deletion in fibroblast was generated. Kidney ischemia/reperfusion injury was employed to induce acute kidney injury in mice.
Results
Here, we found that Rictor/mTORC2 signaling was activated in mouse kidney interstitial fibroblasts after ischemia/reperfusion injury (IRI). Ablation of Rictor in fibroblasts led to more severe kidney dysfunction and morphological damage in mice after IRI compared to their littermate controls. More tubular cell apoptosis and inflammatory cell accumulation were detected in the knockout kidneys after IRI. In in vitro, a co-culture system between fibroblasts and tubular cells was generated. Blockade of Rictor/mTORC2 signaling in fibroblasts with Rictor gene ablation or pharmacological inhibition facilitated staurosporine-induced tubular cell death. In addition, blocking fibroblast mTORC2 signaling could downregulate its peroxisome proliferator-activated receptor gamma (PPARγ) and hepatocyte growth factor (HGF) expression, as well as attenuate c-met signaling activation in tubular cells. Moreover, activation of fibroblast PPARγ could augment HGF expression and attenuate tubular cell death. Notably, in mouse kidneys, ablation of fibroblast Rictor reduced the PPARg and HGF expression. The tyrosine phosphorylation of HGF receptor c-met was also diminished in the knockout kidneys after IRI.
Conclusion
Together, these data suggests that Rictor/mTORC2 signaling activation in kidney interstitial fibroblasts may protect against tubular cell death and dictate the outcome of acute kidney injury through PPARγ-stimulated HGF production.
Funding
- Government Support - Non-U.S.