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Abstract: TH-PO955

The Effect of Complement 3 in Chymase Promoting Renal Interstitial Fibrosis

Session Information

Category: Pathology and Lab Medicine

  • 1501 Pathology and Lab Medicine: Basic

Author

  • Wan, Jian-xin, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
Background

To investigate the effect of complement 3 (C3) on chymase promoting renal interstitial fibrosis.

Methods

To establish UUO model with wild type C57BL/6 mice (WT) and C3 gene knockout mice (C3KO). WT mice were intraperitoneally injected with 10mg/Kg chymostatin in the first to fourteenth day after operation. All mice were sacrificed at fourteenth days and ipsilateral renal tissue. Masson staining was used to observe the renal interstitial fibrosis. The expression of C3, tryptase, chymase, rennin, AngII, TGF-β1, MMP9 in renal interstitium were detected by immunohistochemistry or immunofluorescence or real time quantitative PCR or western blot. Chymase activity of renal tissue was detected by kit. ELISA kit was used to detect AngII, C3a, MMP9.

Results

Compared with WTcontrol group, C3, C3a, mast cells, chymase, renin, AngII, TGF-β1, MMP9 significantly increased in WTuuo group (P<0.001). Compared with WTuuo group, renal tubular injury and interstitial fibrosis were attenuated in C3KOuuo group (P<0.001), C3, C3a, mast cells, chymase, renin, AngII, TGF-β1, MMP9 significantly decreased in WTuuo group (P<0.001). Compared with WTuuo group, enal tubular injury and interstitial fibrosis were attenuated in WTuuo+chymostatin group (P<0.001), chymase activity was significantly decreased (P<0.001), the expression of AngII, TGF-β1 and MMP9 reduced significantly in WTuuo+chymostatin group (P<0.01).

Conclusion

Complement 3 may promote renal interstitial fibrosis by participating in recruitment and activation of mast cells.