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Abstract: TH-PO610

The Effects of Whey Protein Intake on Bone Mineral Metabolism in Rats

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1301 Health Maintenance, Nutrition, and Metabolism: Basic

Authors

  • Kunt, Atilla, Bezmialem Vakif University, Istanbul, Turkey
  • Ozer, Omer faruk, Bezmialem Vakif University, Istanbul, Turkey
  • Toprak, Ali, Bezialem Vakif University, Istanbul, Turkey
  • Kazancioglu, Rumeyza, Bezialem Vakif University, Istanbul, Turkey
Background

High protein intake affects the blood and urine phosphate levels. Phosphate is shown to be correlated with FGF-23. The aim of the study is to investigate the the effects of Whey Protein intake on the whole cascade of bone mineral metabolism.

Methods


48 young male albino wistar rats are divided into 6 (n=8); Normal (20%) Protein(NP)-Sedentary(NPS), NP-Resistance training(NPRT), High(45%)Protein(HP) Sedentary(HPS), HP-Resistance Training(HPRT) , Abuse(70% Protein)(A)-Sedentary(AS), and Abuse-Resistance Training(ART) groups. The rats were fed ad libitum. Training was maintained by resistance protocol in a motorised treadmill.On days 1,30, 60 and 90 a 12 hour urine sample and a blood samples were collected from each animal. Calcium, phospate were detected in the urine.
The blood analysis included: LDH, Albumin, calcium, phosphate. FGF-23 and vit-D was analysed by ELISA.

Results

Results are shown in Table1.

Conclusion

Whey Protein intake negatively affected the the bone mineral metabolism and created a dose-response while training has an alleviating positive effect. Serum, urine phosphate and FGF-23 were shown to be significantly correlated and supports such studies.

Table1
 Normal Whey ProteinHigh Whey ProteinAbuse Whey Proteinp values
SedentaryExerciseSedentaryExerciseSedentaryExerciseDiet EffectTraining EffectDose Effect
 DaysMeanSDMeanSDMeanSDMeanSDMeanSDMeanSD
Serum Albumin (mg/dl)303.630.463.460.923.570.163.390.073.560.123.310.64p<0.05p<0.05p>0.05
603.240.153.440.923.130.073.410.643.150.123.210.11p<0.05p<0.05p<0.05
903.100.122.900.122.600.202.880.132.990.352.650.12p<0.05p<0.05p<0.05
Serum LDH (U/L)30858.25273.95963.38200.611019.14209.611348.29267.751556.25213.351717.88551.35p<0.05p<0.05p>0.05
601466.38248.731021.25331.641358.50419.081195.13196.61472.13387.001471.75416.49p>0.05p>0.05p<0.05
902561.00319.02711.13338.302108.50484.40732.50278.591897.63181.051462.75564.04p<0.05p<0.05p<0.05
Serum Calcium (mg/dl)3010.850.3010.660.3211.080.2610.660.1510.960.2410.930.39p>0.05p>0.05p>0.05
6010.380.3010.280.2510.080.2710.280.1610.440.2910.260.27p>0.05p>0.05p>0.05
9010.180.319.930.139.840.1310.230.7410.010.1010.640.71p>0.05p>0.05p>0.05
Serum Phosphate (mg/dl)306.680.355.760.316.010.645.310.485.860.495.310.47p<0.05p<0.05p>0.05
605.610.605.490.294.790.856.030.425.150.345.910.35p<0.05p<0.05p>0.05
908.891.426.200.848.620.827.211.547.020.637.240.74p>0.05p<0.05p>0.05
FGF-23 (mg/dl)30720.43134.30626.00153.76711.97155.49647.38198.23692.77272.55754.80171.91p>0.05p>0.05p>0.05
60773.29135.32857.89124.12750.39181.18818.26288.27850.8383.66891.7760.14p>0.05p>0.05p>0.05
90913.9074.73636.31203.60942.2373.09889.6193.74885.88136.73806.95120.85p<0.05p<0.05p>0.05
Vitamin D (picogram/dl)304.350.904.190.764.141.365.291.694.280.945.911.63p<0.05p<0.05p>0.05
604.071.904.270.902.950.385.891.503.530.926.162.82p<0.05p<0.05p<0.05
907.174.667.512.776.733.155.972.428.782.8911.503.50p<0.05p<0.05p<0.05
Urine Phosphate (mg/dl)3080.6435.13137.2542.8034.1424.9855.6814.4416.9516.4548.0422.42p<0.05p>0.05p<0.05
6075.8840.8088.2035.0347.5629.7048.8025.7866.7132.5055.8034.90p<0.05p>0.05p<0.05
90115.6465.11103.4945.2862.7477.9382.4053.476.844.72132.00103.69p<0.05p<0.05p<0.05
Urine Calcium (mg/dl)307.711.147.781.7612.692.4614.037.0821.956.6218.542.93p<0.05p>0.05p<0.05
606.781.547.061.0511.153.179.853.0426.819.6627.896.10p<0.05p<0.05p<0.05
908.913.098.331.6613.009.828.632.7623.615.5112.292.40p<0.05p<0.05p<0.05

Funding

  • Clinical Revenue Support