Abstract: TH-PO610
The Effects of Whey Protein Intake on Bone Mineral Metabolism in Rats
Session Information
- Nutrition and Metabolism: Basic
October 25, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1301 Health Maintenance, Nutrition, and Metabolism: Basic
Authors
- Kunt, Atilla, Bezmialem Vakif University, Istanbul, Turkey
- Ozer, Omer faruk, Bezmialem Vakif University, Istanbul, Turkey
- Toprak, Ali, Bezialem Vakif University, Istanbul, Turkey
- Kazancioglu, Rumeyza, Bezialem Vakif University, Istanbul, Turkey
Background
High protein intake affects the blood and urine phosphate levels. Phosphate is shown to be correlated with FGF-23. The aim of the study is to investigate the the effects of Whey Protein intake on the whole cascade of bone mineral metabolism.
Methods
48 young male albino wistar rats are divided into 6 (n=8); Normal (20%) Protein(NP)-Sedentary(NPS), NP-Resistance training(NPRT), High(45%)Protein(HP) Sedentary(HPS), HP-Resistance Training(HPRT) , Abuse(70% Protein)(A)-Sedentary(AS), and Abuse-Resistance Training(ART) groups. The rats were fed ad libitum. Training was maintained by resistance protocol in a motorised treadmill.On days 1,30, 60 and 90 a 12 hour urine sample and a blood samples were collected from each animal. Calcium, phospate were detected in the urine.
The blood analysis included: LDH, Albumin, calcium, phosphate. FGF-23 and vit-D was analysed by ELISA.
Results
Results are shown in Table1.
Conclusion
Whey Protein intake negatively affected the the bone mineral metabolism and created a dose-response while training has an alleviating positive effect. Serum, urine phosphate and FGF-23 were shown to be significantly correlated and supports such studies.
Table1
| Normal Whey Protein | High Whey Protein | Abuse Whey Protein | p values | |||||||||||||
| Sedentary | Exercise | Sedentary | Exercise | Sedentary | Exercise | Diet Effect | Training Effect | Dose Effect | ||||||||
| Days | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||||
| Serum Albumin (mg/dl) | 30 | 3.63 | 0.46 | 3.46 | 0.92 | 3.57 | 0.16 | 3.39 | 0.07 | 3.56 | 0.12 | 3.31 | 0.64 | p<0.05 | p<0.05 | p>0.05 |
| 60 | 3.24 | 0.15 | 3.44 | 0.92 | 3.13 | 0.07 | 3.41 | 0.64 | 3.15 | 0.12 | 3.21 | 0.11 | p<0.05 | p<0.05 | p<0.05 | |
| 90 | 3.10 | 0.12 | 2.90 | 0.12 | 2.60 | 0.20 | 2.88 | 0.13 | 2.99 | 0.35 | 2.65 | 0.12 | p<0.05 | p<0.05 | p<0.05 | |
| Serum LDH (U/L) | 30 | 858.25 | 273.95 | 963.38 | 200.61 | 1019.14 | 209.61 | 1348.29 | 267.75 | 1556.25 | 213.35 | 1717.88 | 551.35 | p<0.05 | p<0.05 | p>0.05 |
| 60 | 1466.38 | 248.73 | 1021.25 | 331.64 | 1358.50 | 419.08 | 1195.13 | 196.6 | 1472.13 | 387.00 | 1471.75 | 416.49 | p>0.05 | p>0.05 | p<0.05 | |
| 90 | 2561.00 | 319.02 | 711.13 | 338.30 | 2108.50 | 484.40 | 732.50 | 278.59 | 1897.63 | 181.05 | 1462.75 | 564.04 | p<0.05 | p<0.05 | p<0.05 | |
| Serum Calcium (mg/dl) | 30 | 10.85 | 0.30 | 10.66 | 0.32 | 11.08 | 0.26 | 10.66 | 0.15 | 10.96 | 0.24 | 10.93 | 0.39 | p>0.05 | p>0.05 | p>0.05 |
| 60 | 10.38 | 0.30 | 10.28 | 0.25 | 10.08 | 0.27 | 10.28 | 0.16 | 10.44 | 0.29 | 10.26 | 0.27 | p>0.05 | p>0.05 | p>0.05 | |
| 90 | 10.18 | 0.31 | 9.93 | 0.13 | 9.84 | 0.13 | 10.23 | 0.74 | 10.01 | 0.10 | 10.64 | 0.71 | p>0.05 | p>0.05 | p>0.05 | |
| Serum Phosphate (mg/dl) | 30 | 6.68 | 0.35 | 5.76 | 0.31 | 6.01 | 0.64 | 5.31 | 0.48 | 5.86 | 0.49 | 5.31 | 0.47 | p<0.05 | p<0.05 | p>0.05 |
| 60 | 5.61 | 0.60 | 5.49 | 0.29 | 4.79 | 0.85 | 6.03 | 0.42 | 5.15 | 0.34 | 5.91 | 0.35 | p<0.05 | p<0.05 | p>0.05 | |
| 90 | 8.89 | 1.42 | 6.20 | 0.84 | 8.62 | 0.82 | 7.21 | 1.54 | 7.02 | 0.63 | 7.24 | 0.74 | p>0.05 | p<0.05 | p>0.05 | |
| FGF-23 (mg/dl) | 30 | 720.43 | 134.30 | 626.00 | 153.76 | 711.97 | 155.49 | 647.38 | 198.23 | 692.77 | 272.55 | 754.80 | 171.91 | p>0.05 | p>0.05 | p>0.05 |
| 60 | 773.29 | 135.32 | 857.89 | 124.12 | 750.39 | 181.18 | 818.26 | 288.27 | 850.83 | 83.66 | 891.77 | 60.14 | p>0.05 | p>0.05 | p>0.05 | |
| 90 | 913.90 | 74.73 | 636.31 | 203.60 | 942.23 | 73.09 | 889.61 | 93.74 | 885.88 | 136.73 | 806.95 | 120.85 | p<0.05 | p<0.05 | p>0.05 | |
| Vitamin D (picogram/dl) | 30 | 4.35 | 0.90 | 4.19 | 0.76 | 4.14 | 1.36 | 5.29 | 1.69 | 4.28 | 0.94 | 5.91 | 1.63 | p<0.05 | p<0.05 | p>0.05 |
| 60 | 4.07 | 1.90 | 4.27 | 0.90 | 2.95 | 0.38 | 5.89 | 1.50 | 3.53 | 0.92 | 6.16 | 2.82 | p<0.05 | p<0.05 | p<0.05 | |
| 90 | 7.17 | 4.66 | 7.51 | 2.77 | 6.73 | 3.15 | 5.97 | 2.42 | 8.78 | 2.89 | 11.50 | 3.50 | p<0.05 | p<0.05 | p<0.05 | |
| Urine Phosphate (mg/dl) | 30 | 80.64 | 35.13 | 137.25 | 42.80 | 34.14 | 24.98 | 55.68 | 14.44 | 16.95 | 16.45 | 48.04 | 22.42 | p<0.05 | p>0.05 | p<0.05 |
| 60 | 75.88 | 40.80 | 88.20 | 35.03 | 47.56 | 29.70 | 48.80 | 25.78 | 66.71 | 32.50 | 55.80 | 34.90 | p<0.05 | p>0.05 | p<0.05 | |
| 90 | 115.64 | 65.11 | 103.49 | 45.28 | 62.74 | 77.93 | 82.40 | 53.47 | 6.84 | 4.72 | 132.00 | 103.69 | p<0.05 | p<0.05 | p<0.05 | |
| Urine Calcium (mg/dl) | 30 | 7.71 | 1.14 | 7.78 | 1.76 | 12.69 | 2.46 | 14.03 | 7.08 | 21.95 | 6.62 | 18.54 | 2.93 | p<0.05 | p>0.05 | p<0.05 |
| 60 | 6.78 | 1.54 | 7.06 | 1.05 | 11.15 | 3.17 | 9.85 | 3.04 | 26.81 | 9.66 | 27.89 | 6.10 | p<0.05 | p<0.05 | p<0.05 | |
| 90 | 8.91 | 3.09 | 8.33 | 1.66 | 13.00 | 9.82 | 8.63 | 2.76 | 23.61 | 5.51 | 12.29 | 2.40 | p<0.05 | p<0.05 | p<0.05 | |
Funding
- Clinical Revenue Support