Kidney Week

Abstract: FR-PO133

The Role of Periostin in Aging Process

Session Information

• Molecular Mechanisms of CKD - II
  October 26, 2018 | Location: Exhibit Hall, San Diego Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

• 1903 CKD (Non-Dialysis): Mechanisms

Authors

• Choi, Young Wook, Seoul National University, Boramae Medical Center, Seoul, Korea (the Republic of)

Young Wook Choi,

• An, Jung Nam, Seoul National University, Boramae Medical Center, Seoul, Korea (the Republic of)

Jung Nam An,

• Kim, Eun Nim, The Catholic University of Korea, Seocho-gu, Seoul, Korea (the Republic of)

Eun Nim Kim,

• Kim, Jinhyuk, SMG-SNU Boramae Medical Center, Seoul, Korea (the Republic of)

Jinhyuk Kim,

• Yang, Seung Hee, Kidney Research Institute, Seoul National University, Seoul, Korea (the Republic of)

Seung Hee Yang,

• Oh, Yun Kyu, Department of Internal Medicine, Boramae Medical Center, Seoul, Korea (the Republic of)

Yun Kyu Oh,

• Lim, Chun Soo, Seoul National University Boramae Medical Center, Seoul, SEOUL, Korea (the Republic of)

Chun Soo Lim,

• Kim, Yon Su, Seoul National University College of Medicine, Seoul, Korea (the Republic of)

Yon Su Kim,

• Choi, Bumsoon, Division of Nephrology, Department of Internal Medicine, Seoul, Korea (the Republic of)

Bumsoon Choi,

• Lee, Jung Pyo, Seoul National University Boramae Medical Center, Seoul, SEOUL, Korea (the Republic of)

Jung Pyo Lee,

Background

Periostin, a matricellular protein, has been reported in diverse processes and pathologies in tissue remodeling through the promotion of adhesion, cell survival, cellular dedifferentiation, and fibrogenesis. However, its role in aging process is unknown.

Methods

We analyzed tissues from 2-months and 24-months old wild-type and Postn null (Postn-/- ) mice and performed high throughput RNA-Sequencing of kidney tissue in aged mice. The genes showing altered expression were confirmed by qRT-PCR analysis.

Results

At 24-months old Postn null mice had preserved kidney tissue and less markers of senescence than wild-type mice. The gross appearance and the kidneys of aged WT mice were bigger and heavier than young WT mice. Serum creatinine levels were also higher in the aged WT mice compared to those in the young WT mice. However, all these changes were diminished in the aged Postn null mice; serum creatinine levels were considerably lower in aged Postn null mice than in aged WT mice. Apparent tubular atrophic changes, interstitial fibrosis, and collagen fiber deposition which were prominent in the aged WT mice than in the young WT mice, were remarkably alleviated in aged Postn null mice. Furthermore, the expressions of periostin were also attenuated in aged Postn null mice compared to in aged WT mice. Also, we found that many of the changes in gene expression that occur during the aging process by periostin.

Conclusion

The data obtained in this study should expand our knowledge on the periostin mediated aging and provide molecular mechanism. Periostin inhibition could have protective effects in aging process.