ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005


The Latest on Twitter

Kidney Week

Abstract: TH-PO512

Detection of Na+ Stores in the Myocardium and Skeletal Muscle of DOCA Treated Mice Using 23Na-MRI

Session Information

Category: Fluid and Electrolytes

  • 901 Fluid and Electrolytes: Basic


  • Dahlmann, Anke, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
  • Linz, Peter, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
  • Perisic, Stojan, Max Planck Institute for medical research, Stuttgart, Germany
  • Tschesche, Jonas, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
  • Nagel, Armin M., Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
  • Titze, Jens, Duke - National University of Singapore, Singapore, Singapore
  • Bäuerle, Tobias, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
  • Kopp, Christoph, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany

Disturbances in Na+ homeostasis with accumulation of Na+ in tissue are present in salt sensitive hypertension. Tissue Na+ distribution could be recently visualized in vivo by 23Na-MRI. If Na+ accumulation occurs also in organs of the cardiovascular system is unknown. We hypothesized that the myocardium is able to absorb significant amounts of Na+ that could be detected by 23Na-MRI.


DOCA-pellets were implanted in 10 male FVB mice while a sham procedure was performed on 10 FVB mice. Subsequently, both groups received 1% NaCl water for 2 weeks. 23Na-MRI at 7Tesla was used to quantify Na+ in heart and skeletal muscle. Furthermore, electrolytes were determined chemically in both tissues. In a fraction of mice intracellular Na+ of the myocardium was measured by electron beam microscopy. Echocardiography was performed and blood pressure determined.


Compared to control mice DOCA treated mice showed a significantly higher Na+ content in skeletal muscle (27.0 ± 5.7 vs. 46.6 ± 8.9 mmol/l, p<0.001) and in heart muscle (61.6 ± 9.1 vs. 73.7 ± 11.7, p<0.05, figure 1). The fraction of bound Na+ was increased in DOCA skeletal muscle (6.4 ± 0.8 vs. 13.0 ± 4.3 a.u., p<0.05) suggesting intracellular Na+ accumulation. Electron beam microscopy of heart muscle also detected a higher intracellular Na+ amount in DOCA animals compared to controls (0.12 ± 0.03 (n=4) vs. 0.29 ± 0.01 a.u. (n=3), p<0.001). Chemical electrolyte analysis confirmed Na+ accumulation in both tissues. A reduced ejection fraction (74 ± 4 vs. 46 ± 15%, p<0.05) and hypertension was found in DOCA animals.


Na+ accumulation occurs intracellularly in skeletal muscle and in heart muscle in vivo upon DOCA salt treatment indicating Na+ uptake rather than extracellular accumulation. Increased Na+ content of the myocardium might directly contribute to cardiac dysfunction.

Figure 1, representative MR images.


  • Government Support - Non-U.S.