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Abstract: SA-OR090

MicroRNA Expression in Human Kidney Allografts Is Associated with Future Development of Fibrosis

Session Information

Category: Transplantation

  • 1801 Transplantation: Basic

Authors

  • Yang, Hua, Weill-Cornell , New York, New York, United States
  • Amrouche, Lucile, Necker hospital , Paris, France
  • Akat, Kemal Marc, The Rockefeller University, New York, New York, United States
  • Li, Carol Y., Weill Cornell Medical College, New York, New York, United States
  • Tinel, Claire, CHU Dijon, Dijon, France
  • Anglicheau, Dany, Necker Hospital, Paris, France
  • Muthukumar, Thangamani, Weill-Cornell , New York, New York, United States
Background

Biomarkers that foretell tubulointerstitial fibrosis (IFTA) of the kidney allograft may help redefine management of kidney transplant recipients.

Methods

We studied 16 kidney allograft surveillance biopsies from 16 recipients at 3 months post–transplantation. All biopsies had Banff ci score (interstitial fibrosis) and ct score (tubular atrophy) of zero. Based on the presence of IFTA in the 12-month surveillance biopsy, 10 recipients were classified as 'Progression to IFTA' (Group A, ci score ≥2 and ct score ≥2) and 6 as 'No Progression to IFTA', (Group B, ci/ct scores=0).

We did RNA sequencing of the 16 biopsies and compared the differential abundance of miRNA between Group A and Group B. We did qRT-PCR assay of the top differentially abundant miRNAs.

Results

Sixteen miRNAs were differentially abundant between the groups; 11 increased and 5 decreased in Group A comapred to Group B (Figure 1). All 16 miRNAs, as reported in prior studies, were associated with organ fibrosis. By qRT-PCR assay, the miRNAs discriminated the groups with high degree of accuracy (Figure 2).

Conclusion

miRNA expression in kidney allograft in 3-month surveillance biopsies is associated with IFTA in 12-month surveillance biopsies.