Abstract: FR-PO1041
The Investigation on the Production of Anti-Neutrophil Cytoplasmic Antibodies in Chronic Bronchitis Rat
Session Information
- Glomerular Diseases: Immunology and Inflammation - II
October 26, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Glomerular Diseases
- 1202 Glomerular Diseases: Immunology and Inflammation
Author
- Xing, Guangqun, The Affiliated Hospital of Qingdao University, Qingdao, SHANDONG, China
Group or Team Name
- Department of Nephrology
Background
The purpose of this study was to investigate the possibility of the production of Anti-Neutrophil Cytoplasmic Antibodies(ANCA) in the rat model of chronic bronchitis with a long-term and recurrent infection.
Methods
The chronic bronchitis model was designed by double stimulation with smoke and lipopolysaccharide(LPS). Rats were divided into four groups, including healthy control rats (group N), group with chronic bronchitis(group CB), group with healthy rats stimulated with PBS and Phorbol-12-mytistate-13-Beetate(PMA)(group N/PBS+PMA), group with chronic bronchitis stimulated with LPS and PMA(group CB/LPS+PMA). Immunological markers, which include Citrullinated Histone H3(CitH3), myeloperoxidase anti-neutrophil cytoplasmic antibodies（MPO-ANCA ) and proteinase 3 anti-neutrophil cytoplasmic antibodies(PR3-ANCA) were measured by enzyme-linked immune sorbent assay(ELISA) at different timepoint after each stimulation. The pulmonary and kidney tissue samples were taken for histopathology. All of related parameters indicators were measured and compared by optimal statistical methods.
Results
(1) The rat models of chronic bronchitis were established and verified by histopathology. (2)With multiple and recurrent stimulation with LPS and/or PMA, the serum levels of CitH3 and MPO-ANCA were found increased gradually. (3)Compared to those in group N, group CB and group N/PBS+PMA, the serum levels of CitH3 in group CB/LPS+PMA were found increased significantly. (4)The serum levels of MPO-ANCA in group N/PBS+PMA and group CB/LPS+PMA were higher than those in group N and group CB. (5)The serum levels of CitH3 in rats were positively correlated with the serum levels of MPO-ANCA（r=0.490, p=0.024). (6)In group with CB/LPS+PMA, mild renal pathology changes could be observed with inflammatory cells infiltration, while no significant functional changes were detected.
Conclusion
With a rat model of chronic bronchitis, recurrent infections combined with multiple stimulation with PMA, MPO-ANCA could be produced.
Funding
- Government Support - Non-U.S.