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Abstract: TH-PO608

Caloric Restriction Improves Established Proteinuria in Adriamycin-Induced Nephropathy

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1301 Health Maintenance, Nutrition, and Metabolism: Basic

Authors

  • Sijbesma, Jürgen, University Medical Center Groningen, Roden, Netherlands
  • Waarde, Aren Van, University Medical Center Groningen, Roden, Netherlands
  • Bakker, Stephan J.L., University Medical Center Groningen, Roden, Netherlands

Group or Team Name

  • 1Dept. of Nuclear Medicine and Molecular Imaging
Background

Reduction of proteinuria is an important strategy to prevent loss of kidney function. Preclinical studies suggest that caloric restriction (CR), induced at young age, protects against age-related proteinuria. None of these studies investigated the effect of CR in established renal disease. We hypothesized that CR in established proteinuria reduces urinary protein excretion (UPE).

Methods

Male Wistar rats (n= 56; age 12± 2 wk) were intravenously injected with 2.1mg/kg Adriamycin. At 6 wks after injection, baseline UPE was measured. At 7 wks after injection rats were randomly assigned to 4 groups: An ad libitum (AL) and a CR group (60% of AL food intake) fed with a 12% protein diet (12%AL, 12%CR) and an AL and a CR group fed with a 20% protein diet (20%AL, 20%CR). All groups were treated for 12 wks. UPE was measured at wk 9 and 12 and mean arterial blood pressure (BP) at the end of the study.

Results

Baseline UPE was similar in all groups (p>0.20) with a median value of 495 and an interquartile range of 127mg/24h. After 12 wks of diet, all animals exposed to CR had a 20.3% lower UPE (p=0.003) compared to AL fed animals. At wk 9 this effect was only seen in the 12%CR group. BP in animals exposed to CR was -21.2% lower (p<0.0001) compared to AL fed animals.

Conclusion

Caloric restriction lowers UPE and BP in rats with established proteinuria. Protein restriction was effective if applied in combination with CR. These results may guide future intervention studies in patients with proteinuric and obesity-related nephropathy.