Abstract: TH-PO608
Caloric Restriction Improves Established Proteinuria in Adriamycin-Induced Nephropathy
Session Information
- Nutrition and Metabolism: Basic
October 25, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1301 Health Maintenance, Nutrition, and Metabolism: Basic
Authors
- Sijbesma, Jürgen, University Medical Center Groningen, Roden, Netherlands
- Waarde, Aren Van, University Medical Center Groningen, Roden, Netherlands
- Bakker, Stephan J.L., University Medical Center Groningen, Roden, Netherlands
Group or Team Name
- 1Dept. of Nuclear Medicine and Molecular Imaging
Background
Reduction of proteinuria is an important strategy to prevent loss of kidney function. Preclinical studies suggest that caloric restriction (CR), induced at young age, protects against age-related proteinuria. None of these studies investigated the effect of CR in established renal disease. We hypothesized that CR in established proteinuria reduces urinary protein excretion (UPE).
Methods
Male Wistar rats (n= 56; age 12± 2 wk) were intravenously injected with 2.1mg/kg Adriamycin. At 6 wks after injection, baseline UPE was measured. At 7 wks after injection rats were randomly assigned to 4 groups: An ad libitum (AL) and a CR group (60% of AL food intake) fed with a 12% protein diet (12%AL, 12%CR) and an AL and a CR group fed with a 20% protein diet (20%AL, 20%CR). All groups were treated for 12 wks. UPE was measured at wk 9 and 12 and mean arterial blood pressure (BP) at the end of the study.
Results
Baseline UPE was similar in all groups (p>0.20) with a median value of 495 and an interquartile range of 127mg/24h. After 12 wks of diet, all animals exposed to CR had a 20.3% lower UPE (p=0.003) compared to AL fed animals. At wk 9 this effect was only seen in the 12%CR group. BP in animals exposed to CR was -21.2% lower (p<0.0001) compared to AL fed animals.
Conclusion
Caloric restriction lowers UPE and BP in rats with established proteinuria. Protein restriction was effective if applied in combination with CR. These results may guide future intervention studies in patients with proteinuric and obesity-related nephropathy.