Kidney Week

Abstract: SA-PO422

Standard Addition Method to Analyze Bisphenols in Dialyzers

Session Information

• Hemodialysis and Frequent Dialysis: Clearance, Technology, Infection
  November 05, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
  Abstract Time: 10:00 AM - 12:00 PM

Category: Dialysis

• 701 Dialysis: Hemodialysis and Frequent Dialysis

Authors

• Haq, Zahin Sultana, Renal Research Institute, New York, New York, United States

Zahin Sultana Haq,

• Wang, Xin, Renal Research Institute, New York, New York, United States

Xin Wang,

• Wang, Xiaoling, Renal Research Institute, New York, New York, United States

Xiaoling Wang,

• Ferreira Dias, Gabriela, Renal Research Institute, New York, New York, United States

Gabriela Ferreira Dias,

• Cheng, Joann, Fresenius Medical Care North America, Waltham, Massachusetts, United States

Joann Cheng,

• Ho, Chih-Hu, Fresenius Medical Care North America, Ogden, Utah, United States

Chih-Hu Ho,

• Breitwieser, Hannah-Madeleine, Fresenius Medical Care Deutschland GmbH, Bad Homburg, Hessen, Germany

Hannah-Madeleine Breitwieser,

• Grobe, Nadja, Renal Research Institute, New York, New York, United States

Nadja Grobe,

• Kotanko, Peter, Renal Research Institute, New York, New York, United States

Peter Kotanko,

Background

Bisphenol A (BPA), a high-volume industrial chemical, has been associated with health risks. Materials made with alternative chemicals such as Bisphenol S (BPS) have been produced, but toxicity concerns were also raised. BPA-containing materials have been used to manufacture dialyzers. Hemodialysis patients’ exposure to BPA/BPS can be measured in dialyzer extractables (E) and leachables (L). However, analytical challenges are E/L complexity, matrix effects, and lack of appropriate blank specimen. We propose Standard Addition Method (SAM) as a good choice for accurate quantification of BPA/BPS in E/L.

Methods

BPA/BPS extracted from dialyzers in exaggerated (95% EtOH, E) and simulated-use (17.2% EtOH, L) conditions were subjected to tandem liquid chromatography-mass spectrometry. For SAM, unspiked E/L and five diluted, authentic BPA/BPS spiked E/L were run. 13C12–labeled BPA/BPS served as internal standards. MassHunter Quantitative Analysis was used for quantification (Fig. 1A).

Results

Concentrations were reported when quality thresholds were met, including repeatable retention time, Q/Q ratio of 100% ± 20, correlation coefficient > 0.99, accuracy and recovery within 100% ± 20. Figure 1B shows two types of dialyzers made from BPA/BPS-containing material (Type A) and BPA/BPS-free material (Type B). With the measured BPA/BPS level in E/L, patient exposure can be estimated.

Conclusion

SAM is a good quantitation method for analyzing BPA/BPS in E/L. Analytical challenges such as matrix complexity and effects and lack of appropriate blank specimen can be overcome. With the measured concentration of BPA/BPS in E/L, patient exposure to BPA/BPS can be estimated, and the toxicological risk assessment on hemodialysis-associated BPA/BPS exposure can be performed.

Figure 1A: BPA/¹³C₁₂ BPA chromatograms and calibration curve for Type A sample. Figure 1B: BPA/BPS levels per device and estimated patient exposure.