Abstract: FR-PO988
The Probiotic Lactiplantibacillus paraplantarum Modulates Serum and Intestinal Environments of Phosphorus in CKD Rats
Session Information
- CKD: Pathobiology - I
November 04, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2203 CKD (Non-Dialysis): Mechanisms
Authors
- Moon, Sung Jin, Catholic Kwandong University International Saint Mary's Hospital, Incheon, Korea (the Republic of)
- Kim, Jwa-kyung, Hallym University, Chuncheon, Gangwon-do, Korea (the Republic of)
- Kim, Hyo Jin, Seoul National University, Gwanak-gu, Seoul, Korea (the Republic of)
Background
The human gastrointestinal tract contains more than 100 trillion microorganisms, including >1000 species of bacteria. Despite many studies linking gut microbes to human diseases, most of the mechanisms by which lactic acid bacteria have beneficial effects on the human body are related to immune modulation. Controlled studies of the ability of lactic acid bacteria to absorb phosphorus directly in the intestine and thereby control serum phosphorus level in in vivo uremic animal models are limited.
Methods
We screened lactic acid bacteria living in Korean fermented foods to identify those that absorb the most phosphorus and noted Lactiplantibacillus paraplantarum KCCM 11826P (Fig 1). Genomic sequencing and CKD animal experiments were performed to explain the mechanism.
Results
That L. paraplantarum strain has a polyP gene cluster, so it absorbs phosphorus better than other bacteria and can suppress strains that produce indole. Supplementing the diets of 5/6 nephrectomized rats with the L. paraplantarum strain significantly decreased serum phosphate level (by 22%) and reduced blood indoxyl sulfate concentration by 40%.
Conclusion
Our results suggest that Lactiplantibacillus preparations could be used for multiple purposes, such as removal of phosphorus and uremic toxins from CKD patients, and demonstrate the novel concept of a probiotic phosphate binder.
Fig 1. Experimental procedure to screen a probiotic strain possessing a high phosphate absorbing ability. Isolation of L. paraplantarum KCCM 11826P by comparison of phosphate consumption.
Funding
- Commercial Support –