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Abstract: TH-PO458

Urinary Single-Cell Sequence Analyzes the Urinary Macrophage in Different Outcomes of Membranous Nephropathy

Session Information

Category: Glomerular Diseases

  • 1302 Glomerular Diseases: Immunology and Inflammation

Authors

  • Liu, Xi, Tongji University School of Medicine, Shanghai, Shanghai, China
  • Yu, Chen, Tongji University School of Medicine, Shanghai, Shanghai, China
Background

Great progresses have been made in diagnosis and treatment of membranous nephropathy, but some patients still do not respond to the immunosuppressive treatment. We performed single-cell sequencing to analysis the urine cells of patients with and without complete remission of MN. Hope to provide some insights for understanding the different outcome of MN.

Methods

Urine single-cell RNA sequencing was performed on 12 healthy controls (HC) and 15 patients with MN. The patients were divided into complete remission group (CR, n=9) and no remission group (NR, n=6).

Results

1) Macrophages were the largest group in urine cells, they were 48.02%, 68.96% and 20.95% in the HC, CR and NR group, respectively . 2) Urinary macrophages expressing FIColin-1 and S100 calcium binding protein A8 were mainly in HC group and CR group, indicating that they were derived from bone marrow and peripheral blood, while the urinary macrophages expressing the regulator of G-protein signaling 1 and HLA-DPA1, mainly in the NR group, were derived from renal resident macrophages. 3) In healthy adults, urine macrophages expressed the metallothionein family, indicating that they can regulate anti-inflammatory and proinflammatory functions bidirectionally. In the CR group, the urine macrophages showed strong proinflammatory properties. In the NR group, the urinary macrophages mainly associated with the level of proteinuria and the impaired renal function (Figure 1).

Conclusion

Our study confirmed the feasibility and effectiveness of urinary single-cell sequencing technology. We firstly delineated urinary cell maps of the MN patients. Not only in origin but also in function of urine macrophages were different in the NC, CR and NR group.

Polarity analysis of urinary macrophages in the CR group and the NR group

Funding

  • Government Support – Non-U.S.