Kidney Week

Abstract: FR-PO470

Fast and Precise Pathogen Detection and Identification in Peritoneal Dialysis-Associated Peritonitis: Insight From Metagenomics Next-Generation Sequencing

Session Information

• Peritoneal Dialysis: Current Topics
  November 04, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
  Abstract Time: 10:00 AM - 12:00 PM

Category: Dialysis

• 702 Dialysis: Home Dialysis and Peritoneal Dialysis

Authors

• Ma, Xiaoxiao, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Xiaoxiao Ma,

• Ha, Xiaowen, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Xiaowen Ha,

• Abudula, Reziwanguli, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Reziwanguli Abudula,

• Azati, Jiayinaxi, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Jiayinaxi Azati,

• Wu, Yaqin, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Yaqin Wu,

• Tan, Yixiang, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Yixiang Tan,

• Jiang, Hong, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China

Hong Jiang,

Background

PD-associated peritonitis (PDAP) is one of the most common and detrimental complications for patients receiving PD.Metagenomics next-generation sequencing technique (mNGS) potentially provides a fast and precise option for the detection and identification of pathogens in the PDAP by sequencing the nucleic acid of microorganisms in the samples.

Methods

We investigated 12 PDAP patients by identifying the potential pathogens of the peritoneal dialysate samples using the traditional microorganism culture(TMC) and mNGS respectively. The pathogens detection rate, species coverage of pathogens, sensitivity, and specificity between these two assays were analyzed.

Results

Total concordance between TMC and mNGS was noted. The pathogenic bacteria detection rate using mNGS was significantly higher than that of TMC (75% vs 58.3%, P<0.05). The rates for greater than 2 pathogens using mNGS dramatically increased than that of TMC (91.7% vs 58.3, 41.4% vs 0%, P<0.05). The sensitivity and specificity of mNGS for the detection of pathogens in the PDAP are higher than that of TMC. Of note, the TMC results from three PDAP patients showed negative due to previous antibiotics treatment before enrolling in this study, whereas positive pathogens were still detected by using mNGS. The adjustment of treatment according to the result of mNGS controlled the infection, 2 patients and peritoneal dialysis was achieved independently after 1 week. The time of results was 5-7 days for TMC and 1-1.5 days for mNGS.The sensitivity of mNGS is 70% and the specificity of it is 100%.

Conclusion

These findings suggest that mNGS in diagnosis and guidance of treatment for PDAP is possibly superior to TMC its higher sensitivity and specificity.

Funding

• Government Support – Non-U.S.