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Kidney Week

Abstract: SA-PO089

Effect of Ischemia-Reperfusion Injury on Nuclear Malondialdehyde Levels in Kidney Cortex and Medulla of Aged Rats

Session Information

  • AKI: Mechanisms - III
    November 05, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Zamlauski-Tucker, Marianna J., Ball State University, Muncie, Indiana, United States
  • Ye, Bingwei, Ball State University, Muncie, Indiana, United States
Background

The present study was undertaken to determine the effect of ischemia-reperfusion injury (IRI) on nuclear malondialdehyde (MDA) levels in kidneys from aged rats. MDA is a product of lipid peroxidation of cell and organelle membranes by free radicals and is used as an indicator of oxidative stress. Kidney dysfunction is associated with tissue damage caused by free radicals generated in IRI.

Methods

Anesthetized old female Lewis rats (22 months of age) were used in the study. The left and right renal pedicles were clamped for 60 min, followed by 60 min of reperfusion in the Experimental Group (n =6). The kidneys were then harvested, separated into cortex and medulla, and homogenized. Kidneys in the Control Group (n =6) were not subjected to IRI before being harvested. The nuclear fractions were isolated using differential centrifugation, and the MDA levels were measured using a spectrophotometric assay. The water contents of the cortex and medulla were determined to allow MDA to be expressed as nmol/g kidney dry wt. A Student's T Test was used to compare the data, and statistical significance was determined at p < 0.05. All data reported as X ± SEM.

Results

Nuclear MDA levels were significantly decreased in the kidney cortex of the Experimental Group when compared to the Control group. Nuclear MDA levels in kidney cortex decreased by 49 % ,with Nuclear MDA being 36.5 ± 7.9 % nmol/g kidney dry wt in the Control Group and 18.0 ± 1.7 nmol/g kidney dry wt in the Experimental Group. Nuclear MDA levels in the kidney medulla were not different with IRI. Nuclear MDA levels were 45.0 ± 5.9 nmol/g kidney dry wt in the Control Group and 52.5 ± 2.6 nmol/g kidey dry wt in the Experimental Group.

Conclusion

The results suggest that in IRI, the nucleus in both the kidney cortex and medulla of aged rats may not be experiencing increased oxidative stress and damage, as indicated by a decrease or no change in MDA levels, respectively, after 60 min of reperfusion.