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Abstract: FR-PO794

Longitudinal Analysis of Donor-Derived Cell-Free DNA (dd-cfDNA) in En Bloc Kidney Transplantation

Session Information

Category: Transplantation

  • 2002 Transplantation: Clinical

Authors

  • Akkina, Sanjeev, Loyola University Chicago, Chicago, Illinois, United States
  • Singh, Neeraj, Willis-Knighton Health System, Shreveport, Louisiana, United States
  • Klein, Jeffrey A., University of Kansas School of Medicine, Kansas City, Kansas, United States
  • Agrawal, Nikhil, CareDx Inc, Brisbane, California, United States
  • Fei, Mingwei, CareDx Inc, Brisbane, California, United States
  • Desai, Amishi S., Loyola University Chicago, Chicago, Illinois, United States
Background

The longitudinal dd-cfDNA patterns in en-bloc kidney transplant recipients have not been studied. It is unknown how the initial smaller mass of the two pediatric organs impacts ddcfDNA early post-transplant or if the dd-cfDNA rises over time as the organs enlarge. We analyzed ddcfDNA scores over thefirst 12 months in recipients of pediatric en-bloc kidneys enrolled in the Kidney allograft Outcomes AlloSure Registry (KOAR, NCT03326076).

Methods

16 recipients of pediatric en-bloc kidneys with donors aged ≤7 years old were identified and compared with 931 single, non en-bloc deceased donorrecipients. Patients with rejection were excluded.

Results

Of the 16 en-bloc recipients, 56.2% were male with median donor age of 1.4 years and median cold ischemia time (CIT) of 20 hours. None of the en-bloc transplants had delayed graft function. Aside from KDPI, donor age, and recipient age, no other differences were observed between groups.
Higher median ddcfDNA values were seen in the en-bloc cohort compared to not en-bloc at month 1 (M1, 1.4% vs 0.4%) and month 2 (0.58% vs 0.23%) after transplant (p<0.001) [Figure 1a]. Median ddcfDNA values were comparable for month 3 (0.21% vs 0.16%, p = 0.07) and all subsequent time points. [Figure 1a,1b]. Among 8 patients with M1 ddcfDNA results, there was no significant association between M1 ddcfDNA and either CIT (r=0.33, p=0.419) or KDPI (r=0.23,p=0.589).
.

Conclusion

Our results demonstrate higher early post-transplant ddcfDNA values among en-bloc kidney compared to single kidney recipients however, no difference or upward trend is observed beyond month 2 and out to 12 months.

Funding

  • Commercial Support