ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on Twitter

Kidney Week

Abstract: TH-PO095

Dietary Modification of Fat and Protein Composition and Its Effects on the Repair of Ischemic AKI

Session Information

  • AKI: Mechanisms - I
    November 03, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Jeon, Junseok, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Lee, Yunsoo, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Seo, Minsuk, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Lee, Kyungho, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Lee, Jung eun, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Huh, Wooseong, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Kim, Yoon-Goo, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
  • Jang, Hye Ryoun, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
Background

The effects of dietary composition on the repair after ischemic acute kidney injury (AKI) are not well established. The effects of dietary modification of fat and protein composition on intrarenal immunologic micromilieu and the repair of tubular damage after ischemic AKI were investigated using murine ischemic AKI and human kidney-2 (HK-2) cell hypoxia model.

Methods

Three different diet regimen were provided to mice (9-week-old male C57BL/6) from day 3 after bilateral or unilateral ischemia-reperfusion injury (BIRI or UIRI) operation: control diet, high-fat with high-protein (HF+HP) diet, and low-fat with low protein (LF+LP) diet. HK-2 cells were treated with additional lipid or amino acid after hypoxic insult.

Results

Body weight was greater and total cholesterol concentration was higher in the HF+HP group, and body weight was lesser and blood pressure and BUN were lower in the LF+LP group compared to the control diet group, while there was no difference in plasma creatinine between groups. In the expression of intrarenal cytokines/chemokines, RANTES was increased in the HF+HP group, and IFN-γ, IL-4, IL-10, IL-6, TNF-α were increased and VEGF was decreased in the LF+LP group than in the control group after BIRI. IFN- γ, IL4, and IL-6 were decreased in the HF+HP group and IL-6 was increased in the LF+LP group than in the control group after UIRI. The fibrosis of postischemic kidney was more extensive in the LF+LP group compared to the control group after UIRI, while comparable between groups after BIRI. In HK-2 cell hypoxia model, addition of amino acid suppressed, but addition of lipid promoted proliferation of HK-2 cells after hypoxic insult.

Conclusion

Excessive restriction of protein and fat during the healing phase of ischemic AKI can be detrimental. Further studies are needed to clarify the optimal dietary compositions and the individual effects of protein and lipid during the recovery of ischemic AKI.

Funding

  • Government Support – Non-U.S.