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Kidney Week

Abstract: SA-PO102

Canagliflozin Improves Renal Oxidative Stress and Inflammation in Ischemia-Reperfusion Syndrome in Rats

Session Information

  • AKI: Mechanisms - III
    November 05, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Vattimo, Maria de Fatima F., Universidade de Sao Paulo, Sao Paulo, Brazil
  • Ventura, Sara, Universidade de Sao Paulo, Sao Paulo, Brazil
  • Silva, Eloiza Oliveira, Universidade de Sao Paulo, Sao Paulo, Brazil
  • Debbas, Victor, Universidade de Sao Paulo, Sao Paulo, São Paulo, Brazil

Group or Team Name

  • Experimental Research Group on Acute Kidney Injury
Background

Recent studies have shown that sodium-glucose cotransporter 2 (SGLT-2) inhibitors alleviate acute kidney injury (AKI) in diabetic patients. Ischemia-reperfusion (I/R) syndrome is the most relevant cause of AKI. Several molecular mechanisms, including oxidative stress as well as the inactivation of anti-inflammatory pathways such as Nrf2, contribute to the I/R syndrome. The aim of this study is to investigate the renoprotective effect of canagliflozin on I/R syndrome in non-diabetic rats.

Methods

Wistar rats were divided into: SHAM: surgery control; I/R: ischemic group (30 minute bilateral renal clamping); CANA: canagliflozin (30 mg/kg, once, daily, 5 days); CANA+I/R: as described. Renal hemodynamics such as renal blood flow (RBF) and renal vascular resistance (RVR); renal function (inulin clearance, plasma creatinine); oxidative metabolites (urinary peroxides, TBARS, urinary nitrate and thiols in renal tissue) and Nrf2 were analyzed.

Results

[Figure 1] [Figure 2]

Conclusion

Canagliflozin did not induce hypoglycemia and has significant potential as a therapeutic intervention to ameliorate renal injury after renal I/R and attenuate oxidative stress and inflammation.

Figure 1. Hemodynamics, renal function and oxidative estress.
Values shown are mean±SE: a p<0,05 versus SHAM; b p<0,05 versus CANA; c p<0,05 versus I/R were significantly different as indicated; one way ANOVA followed by Tukey's pos test were performed.

Figure 2. Densitometry of Nrf2. I/R syndrome can inhibit the Nrf2 pathway.

Funding

  • Government Support – Non-U.S.