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Abstract: FR-PO529

A Retrograde Implantation Approach for Peritoneal Dialysis Catheter Placement in Mice

Session Information

Category: Dialysis

  • 702 Dialysis: Home Dialysis and Peritoneal Dialysis


  • Sellinger, Isaac E., Boston University School of Medicine, Boston, Massachusetts, United States
  • Lotfollahzadeh, Saran, Boston University School of Medicine, Boston, Massachusetts, United States
  • Stern, Lauren D., Boston University School of Medicine, Boston, Massachusetts, United States
  • Francis, Jean M., Boston University Renal Section, Boston, Massachusetts, United States
  • Chitalia, Vipul C., Boston University Renal Section, Boston, Massachusetts, United States
  • Belghasem, Mostafa, Kaiser Permanente Bernard J Tyson School of Medicine, Pasadena, California, United States

Chronic kidney disease (CKD) has reached pandemic proportions. Almost one in seven and two in 1,000 Americans have CKD, and End-Stage Kidney Disease (ESKD), respectively. While hemodialysis is the mainstay of renal replacement therapy, peritoneal dialysis (PD) provides distinct advantages. Murine models are employed to investigate details of peritoneal membrane failure. Current murine models suffer from technical challenges like catheter migration and kinking that often warrant early catheter removal and compromise the models’ performance. We set out to improve a PD murine model and overcome these key limitations.


Ten C57BL/6J mice, 8–12 weeks in age, were used. Peritoneal catheters were placed with a novel retrograde technique. This method was validated using an LPS-induced inflammation assay. LPS was injected with 2 mg/kg bodyweight for 7 days. The peritoneal membrane was probed for fibrosis, neovascularization, and sub-peritoneal space using integrated density measurements normalized to the area.


A retrograde method of PD catheter was adopted. Accordingly, the catheter was customized with a side hole and retrograde tube passing through a prefabricated tract. All the implanted catheters were functional till the end of the study. No incidences of catheter dislodgement, leaking of PD fluid, skin damage, or catheter kinking were noted. Compared to PBS, LPS exposure resulted in a significant increase in the extracellular matrix in the sub-peritoneal space(P=0.008), a 3-fold increase in sub-peritoneal fibrosis (P=0.015), and an 8–9-fold increase in the vascularity (P=0.0168).


Out of 3 conventional murine models of PD, we now describe a modified method that significantly improved the PD catheter functions and avoided all the complications noted with the previous method. The current modifications will now pave the way to generate a durable murine model to investigate the long-term consequences of peritoneal membrane failure in human ESKD patients.


  • NIDDK Support