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Abstract: TH-PO639

Changes in Urinary Proteome in Living Kidney Donors After Kidney Donation

Session Information

  • Transplantation: Basic
    November 03, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
    Abstract Time: 10:00 AM - 12:00 PM

Category: Transplantation

  • 2001 Transplantation: Basic

Authors

  • Naik, Sachin Motiram, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India
  • Kamboj, Kajal, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India
  • Kaur, Jaskiran, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India
  • Bharati, Joyita, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India
  • Yadav, Ashok Kumar, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India
  • Kumar, Vivek, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India
Background

Recent concerns about long term effects of living kidney donation have generated interest in adaptive mechanisms post kidney donation. We present initial data on changes in urinary proteome over 6 months after living kidney donation.

Methods

Prospective living kidney donors, scheduled for living donor kidney transplantation, were enrolled. Participants were followed up at 6 months after surgery. Urine samples for proteomics were collected before surgery and at 6 months after surgery. Urinary proteomic profiling was done by liquid chromatography and mass spectrometry (LC-MS/MS) using Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer.

Results

25 participants have been enrolled till date. We present data of 15 participants who have completed follow up. The mean age was 43.2±7.62 years. Serum creatinine increased while hemoglobin and serum inorganic phosphorus decreased at 6 months after living kidney donation. Proteomic analyses showed 257 differentially expressed proteins (DEPs) (115 downregulated, 142 upregulated, figure 1). Gene ontology enrichment analysis suggest upregulation of insulin like growth factor pathway, growth factor activity, cell junction organization and cell activation, and downregulation of generation of precursor metabolites and energy, various solute transporters, cell cycle, cell adhesion molecule binding, MAPK family signaling pathway. The protein-protein interactions and pathway relatedness suggest linkage to Erb1 downstream pathway, Rho GTPase effectors, PDGFRB pathway etc.

Conclusion

This study provides clues to possible biological processes and pathways involved in kidney adaptation. However, these need more evaluation and validation.

Selected upregulated and downregulated proteins

Funding

  • Government Support – Non-U.S.