Abstract: FR-PO0371
Mobilization of Antioxidant Vitamin Reserves to Combat Oxidative Stress in Cardiorenal Syndrome Due to Right Ventricular Failure
Session Information
- AKI: Mechanisms - Metabolism and Repair
October 23, 2026 | Location: Exhibit Hall A, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Author
- Farahmand, Firoozeh, Saint Louis University, St. Louis, Missouri, United States
Background
Cardiorenal syndrome (CRS) due to right ventricular (RV) failure is a key indicator of poor prognosis, significantly increases mortality in patients with multiple organ dysfunction. Targeting molecular mechanisms is crucial for effective therapy. Present study investigates antioxidant vitamin E concentrations in the plasma, heart and kidney with or without CRS. The effects of dietary vitamin E supplementation for 4 weeks on CRS, vitamin E status, as well as hemodynamic function and histology in these groups, were also examined..
Methods
Rats were randomized into 4 groups:Control, ALK induced CRS due to RVF, CRS + Commercial Diet, and CRS + Vitamin E (2 weeks pre/post-induction). Daily observations included monitoring for dyspnea, cyanosis. At 4 wks they were examined for hemodynamic function including pulmonary artery acceleration time (PAAT),RV systolic pressure (RVSP), RV diastolic pressure (RVDP), RV hypertrophy (RVH), and RV function. Heart, kidney, and plasma were analyzed for antioxidant and lipid peroxidation (LPX) as well as antioxidant enzyme activities including superoxide dismutase ( SOD) glutathione peroxidase (GSHPx), catalase and oxidative stress. After sacrificing the animals, the renal cortex and the heart were removed for histology.
Results
At 4 weeks, the CRS group on a control diet demonstrated significantly higher RVSP and lower PAAT, accompanied by elevated renal and myocardial LPX, decreased activity of antioxidant enzymes.and acute tubular necrosis (ATN).Vitamin E plasma concentrations did not differ between the CRS and sham control groups,but concentrations in the RV and kidney were significantly decreased in the CRS group. Vitamin E supplementation improved hemodynamic function, increased vitamin E concentrations in plasma, RV and kidney,. Kidney histopathology with Periodic acid-Schiff (PAS) staining demonstrated ATN. Vit E supplementaion prevented ALK-induced CRS and decreased oxidative stress in RV and the kidney
Conclusion
This study suggests for the first time that changes in RV and kidney Vitamin E concentrations are better indicator of myocardial and renal oxidative stress compared with plasma concentration.Dietary supplementation with vitamin E, increased storage-organ including kidney concentrations of vitamins E, prevented ATN and improved RV function. Antioxidants supplemenations may play a role as adjunct therapy in CRS.