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Abstract: FR-PO185

A Whole Blood Adsorber Particle for Future Applications in the Treatment of CKD Patients

Session Information

Category: CKD (Non-Dialysis)

  • 1901 CKD (Non-Dialysis): Epidemiology, Risk Factors, and Prevention

Authors

  • Sternkopf, Marieke, University Hospital Aachen, Aachen, Germany
  • Noels, Heidi, RWTH University Aachen, Aachen, Germany
  • Jankowski, Vera, University hospital RWTH Aachen, Aachen, Germany
  • Jankowski, Joachim, RWTH Aachen, Aachen, BE, Germany
Background

Hydrophobic uremic toxins accumulate in patients with chronic kidney disease, contributing to a highly increased cardiovascular risk. The clearance of these uremic toxins by current hemodialysis techniques is limited due to their high binding affinity to plasma proteins, which prevents their removal through the pores of dialysis membranes. Adsorber techniques may be an appropriate alternative and/or additional technique to increase hydrophobic uremic toxin removal.

Methods

We developed an extracorporeal adsorber particle for efficient adsorption of these uremic toxins. The whole blood adsorber particle consists of a porous, activated charcoal core with a hydrophilic surface coating of cross-linked polyvinylpyrrolidone. The adsorption capacity of the particles was quantified by analytical chromatography after incubation or perfusion of the particles with an aqueous serum albumin solution or blood, each containing mixtures of hydrophobic uremic toxins.

Results

The particle hemocompatibility was assessed by quantifying the production of thrombin-antithrombin III complex (TAT) and complement component 5a (C5a) as well as leukocyte and thrombocyte counts in blood. A time-dependent increase in hydrophobic uremic toxin adsorption was depicted and all tested toxins showed a high binding affinity to the adsorber particles. Further, the particle showed a good hemocompatibility without significant effects on C5a, TAT or thrombocyte concentration, although leukocyte counts were slightly reduced

Conclusion

In conclusion, the whole blood adsorber particle is hemocompatible and shows a high adsorption capacity towards hydrophobic uremic toxins. Thus, it is an interesting candidate for further in vivo studies with the aim to increase the efficiency of conventional dialysis techniques and reduce mortality in CKD patients.