Abstract: SA-PO594
Heme Oxigenase-1 (HO-1) and Inducible Nitric Oxide Synthase (iNOS) Modulate by Resveratrol in Contrast-Induced AKI in CKD Rats
Session Information
- AKI: Other Mechanisms and Cell Cultures
October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Fernandes, Sheila Marques, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil
- Vattimo, Maria De Fatima, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil
- Watanabe, Mirian, University of Sao Paulo, Sao Paulo, Brazil
- Peres, Karina Batista, EEUSP, São Paulo, Brazil
- Martins, Daniel Malisani, University of São Paulo, Carapicuíba, Brazil
- Vasco, Carolina Ferreira, Universidade de São Paulo, São Paulo, Brazil
Group or Team Name
- Experimental lab on animal models
Background
Iodinated contrast (IC)-induced acute kidney injury (AKI) is an important complication in patients with chronic kidney disease (CKD). In previous studies, we demonstrated a protective effect of Resveratrol (R) treatment demonstrated improvement of renal function in IC-AKI by renal hemodynamic modulation in CKD rats (VATTIMO MFF, JASN 2016). The aim of this study was to evaluate the potential renoprotective effect of resveratrol as anti-oxidate and anti-nitrosative by modulation of heme oxygenase-1 (HO-1) and nitric oxide induced synostosis (iNOS) in rats with CKD treated with IC.
Methods
Wistar, adult, male rats were randomized in four groups. Sham (control group), 5/6 (CKD model: 5/6 nefrectomy), 5/6+IC (CKD model + IC, 6ml/Kg, intraperitoneal- i.p., single dose), 5/6+IC+R (CKD model + R, 25 mg/Kg, intraperitoneal, single dose + IC, i.p, single dose, 30 minutes after R). Gene expression and protein syntesis of HO-1 and iNOS in renal tissue were evaluated.
Results
Treatment with IC significantly incresed gene expression and protein synthesis of HO-1 in 5/6 + IC rats compared to the 5/6 (DRC model) group and treatment with Resveratrol induced an additional elevation in gene expression and in the protein synthesis of HO-1 in 5/6 + IC + R rats. The levels of gene expression and protein synthesis of iNOS also to increase in the kidney of 5/6+IC group. 5/6 + IC + R reduced iNOS protein levels compared to 5/6 + IC rats.
Conclusion
These results demonstrate that Resveratrol influence renal HO-1 and iNOS expression after IC-induced AKI in CKD rats and may contribute to better outcomes in this setting by involvement in the modulation of oxidative and nitrosative stress.
Funding
- Government Support - Non-U.S.