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Abstract: SA-PO594

Heme Oxigenase-1 (HO-1) and Inducible Nitric Oxide Synthase (iNOS) Modulate by Resveratrol in Contrast-Induced AKI in CKD Rats

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Fernandes, Sheila Marques, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil
  • Vattimo, Maria De Fatima, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil
  • Watanabe, Mirian, University of Sao Paulo, Sao Paulo, Brazil
  • Peres, Karina Batista, EEUSP, São Paulo, Brazil
  • Martins, Daniel Malisani, University of São Paulo, Carapicuíba, Brazil
  • Vasco, Carolina Ferreira, Universidade de São Paulo, São Paulo, Brazil

Group or Team Name

  • Experimental lab on animal models
Background

Iodinated contrast (IC)-induced acute kidney injury (AKI) is an important complication in patients with chronic kidney disease (CKD). In previous studies, we demonstrated a protective effect of Resveratrol (R) treatment demonstrated improvement of renal function in IC-AKI by renal hemodynamic modulation in CKD rats (VATTIMO MFF, JASN 2016). The aim of this study was to evaluate the potential renoprotective effect of resveratrol as anti-oxidate and anti-nitrosative by modulation of heme oxygenase-1 (HO-1) and nitric oxide induced synostosis (iNOS) in rats with CKD treated with IC.

Methods

Wistar, adult, male rats were randomized in four groups. Sham (control group), 5/6 (CKD model: 5/6 nefrectomy), 5/6+IC (CKD model + IC, 6ml/Kg, intraperitoneal- i.p., single dose), 5/6+IC+R (CKD model + R, 25 mg/Kg, intraperitoneal, single dose + IC, i.p, single dose, 30 minutes after R). Gene expression and protein syntesis of HO-1 and iNOS in renal tissue were evaluated.

Results

Treatment with IC significantly incresed gene expression and protein synthesis of HO-1 in 5/6 + IC rats compared to the 5/6 (DRC model) group and treatment with Resveratrol induced an additional elevation in gene expression and in the protein synthesis of HO-1 in 5/6 + IC + R rats. The levels of gene expression and protein synthesis of iNOS also to increase in the kidney of 5/6+IC group. 5/6 + IC + R reduced iNOS protein levels compared to 5/6 + IC rats.

Conclusion

These results demonstrate that Resveratrol influence renal HO-1 and iNOS expression after IC-induced AKI in CKD rats and may contribute to better outcomes in this setting by involvement in the modulation of oxidative and nitrosative stress.

Funding

  • Government Support - Non-U.S.