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Abstract: TH-PO613

Effect of Exogenous Supplementation with Alpha Lipoic Acid on Nuclear Reduced Glutathione Levels in Rat Kidney Cortex and Medulla

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1301 Health Maintenance, Nutrition, and Metabolism: Basic

Authors

  • Zamlauski-Tucker, Marianna J., Ball State University, Muncie, Indiana, United States
  • Ye, Bingwei, Ball State University, Muncie, Indiana, United States
Background

Dietary supplementation with the antioxidant alpha lipoic acid has been found to increase reduced glutathione (GSH) levels in mitochondria from kidney and heart tissue in old rats. GSH is the major antioxidant inside cells and provides protection against damage by free radicals produced as a consequence of oxidative metabolism. The purpose of this study was to investigate the effect of dietary supplementation with alpha lipoic acid on GSH levels in the nucleus of rat kidney cells.

Methods

Old female Experimental Lewis rats (22 months of age; n = 4) received alpha lipoic acid (100 mg/Kg of body wt) via i.p. injection for one week. Age-matched Control rats (n = 4) did not receive any supplementation. The kidneys were harvested from anesthetized rats and the cortex and medulla were separated and homogenized. The nuclear fractions were isolated using differential centrifugation. The GSH and total glutathione (Tot GLUT; GSH plus oxidized GSH ) were measured using a spectrophotometric assay. GSSG (oxidized GSH) levels were estimated from the difference between Tot GLUT and GSH levels and then divided by 2. Comparisons were done using a Student' s T Test.

Results

There were significant increases in GSH and Tot GLUT levels in both the kidney cortex and medulla with dietary supplementation.

Conclusion

These findings suggest that dietary supplementation with alpha lipoic acid may also be beneficial to cell nuclei in rat kidney by increasing the GSH levels and thus, providing protection against free radical damage.

Effect of Alpha Lipoic Acid on Nuclear GSH Levels in the Rat Kidney
 GSH - nmol/ g kid wet wtGSSG - nmol/ g kid wet wtTot GLUT - nmol/ g kid wet wt
 ControlExperimentalControlExperimentalControlExperimental
Cortex112 ± 17245 ± 44 *2.1 ± 0.25.5 ± 1.5116 ± 16258 ± 41 *
       
Medulla99 ± 10219 ± 42 *2.9 ± 0.511.7 ± 5.6105 ± 9310 ± 50 *
       

All data shown as X +/- SEM; * Significantly different from Control at p < 0.05