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Abstract: TH-PO626

Chondroitin Sulfate Supplement Alleviates Nephrocalcinosis in Hyperoxaluric Rats

Session Information

Category: CKD (Non-Dialysis)

  • 1902 CKD (Non-Dialysis): Clinical, Outcomes, and Trials

Authors

  • Dissayabutra, Thasinas, Chulalongkorn University, Pathumwan, Bangkok, Thailand
  • Kalpongnukul, Nuttiya, Faculty Of Medicine, Chulalongkorn University, Pathumwan, Bangkok, Thailand
  • Chindaphan, Kanokporn, Chulalongkorn University, Pathumwan, Bangkok, Thailand
  • Ungjaroenwathana, Wattanachai, Sunpasitthiprasong Hospital, Meung, Ubon Ratchathani, Thailand
  • Kaewwongse, Maroot, University of Phayao, Phayao, Thailand
  • Iampenkhae, Kroonpong, King Chulalongkorn Memorial Hospital, Bangkok, Thailand
  • Tosukhowong, Piyaratana, Chulalongkorn University, Pathumwan, Bangkok, Thailand
Background

Nephrocalcinosis and nephrolithiasis are common features of primary hyperoxaluria that contributes to end stage renal disease. We observed low urinary chondroitin sulfate (CS) and high hyaluronic acid (HA) excretion in hyperoxaluric nephrolithiasis patients and considered these were risks of nephrocalcinosis. This study aimed to investigate the effect of CS and HA supplement in hyperoxaluric rats.

Methods

Hyperoxaluric Wistar rats induced by ethylene glycol and vitamin D intraperitoneal injection was supplemented with CS (40 mg/kg/day) and/or HA (10 mg/kg/day). Urinary CS and HA excretion were measured. Histopathology of kidney tissue was reported.

Results

All hyperoxaluric rats developed moderate to severe nephrocalcinosis in 15 days. CS supplement entirely inhibited calcium deposition in renal tissue in all rats (p < 0.05). HA supplement had no effect on nephrocalcinosis formation, however, combination of CS and HA supplement can lessen the degree of nephrocalcinosis, but not as effective as giving CS alone.

Conclusion

CS supplement in hyperoxaluric patients could be beneficial in prevention of nephrocalcinosis. Besides, HA supplement might have detrimental effect in interrupt the CS activity.

Severity score of nephrocalcinosis in hyperoxaluric rats
GroupNephrocalcinosis grade (Median, IQR)Tubular obstructionTubulo-interstitial fibrosis
Control0 (0)00
Hyperoxaluria4 (2-4)00
CS supplement0 (0)*00
HA supplement2.5 (1-4)00
CS & HA supplement0.5 (0-2)*00

Nephrocalcinosis grading score: 0, no deposit; 1, 1-2 deposits/section; 2, 3-5 deposit/section; 3, 5-10 deposits/section; 4, >10 deposits/section. Tubular obstruction score: 0, no obstruction; 1, tubular obstruction present. Tubulo-interstitial fibrosis: 0, no fibrosis; 1, fibrosis present. *p < 0.05 compared with Hyperoxaluria group.