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Abstract: TH-PO644

BMSCs Labeled with Sialyl Lewis X Had Higher Homing Rate to the Kidney of AKI Rats

Session Information

Category: Development, Stem Cells, and Regenerative Medicine

  • 501 Development, Stem Cells, and Regenerative Medicine: Basic

Authors

  • Yang, Lin, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi, China
  • Wang, Qi, Nanchang University, Graduate School, Nanchang, China
  • Luo, Lihua, Ganzhou People's Hospital, Ganzhou, JIangxi, China
  • Luo, Fuli, Jiangxi University of Traditional Chinese Medicine?University Hospital, Nanchang, China
  • Li, Yun, Jiangxi Provincial Peoples'' Hospital, Nanchang, China
Background

Bone marrow mesenchymal stem cells (BMSCs) were applied to treat various diseases. After transplantation, BMSCs could migrate to the injured organ which is called homing. To enhance homing, a new technology has been developed, in which biotin-streptavidin is used to attach sialyl LewisX, the ligand of adhesion molecule to BMSCs surface.

Methods

BMSCs from male SD rats were isolated, amplified, and labeled with sialyl LewisX by biotin-streptavidin system. The acute kidney injury(AKI) rat model was induced by cisplatin in female SD rats. Forty-eight AKI rats were divided into three groups: PBS, labeled BMSCs, and unlabeled BMSCs. After application of cisplatin, rats were transplanted with BMSCs or PBS.

Results

BMSCs significantly improved the renal function of AKI rats. Serum creatinine and blood urea nitrogen in BMSCs groups were statistically significantly lower than those of PBS group on 4-7 days after cisplatin injection (Fig. 1,2). Unfortunately the differences of renal function between the labeled and unlabeled groups were not statistically significant.
There were more cells exuded in renal glomeruli and interstitium in the labeled group than in the unlabeled or PBS group, suggesting that the BMSCs labeling may increase BMSCs' homing to the kidney of AKI rats (Fig. 3-5). BMSCs reduced renal tubular and interstitial lesions to some extent, such as renal tubular dilatation, epithelial cell detachment, basement membrane exposure, intra-luminal cellular cast, interstitial edema and cell infiltration. The regeneration of renal tubular epithelium in transplanted groups were slightly higher (Fig. 6-8).

Conclusion

BMSCs labeled with sialyl LewisX had higher homing rate to the kidney of AKI rats. BMSCs transplantation significantly improved the renal function of AKI rats, and to some extent, reduced renal tubular and interstitial lesions.

Funding

  • Government Support - Non-U.S.