Kidney Week

Abstract: FR-PO871

Urinary Exosomal MicroRNA as a Non-Invasive Biomarker for the Diagnosis of Acute Rejection in Kidney Transplant Recipients

Session Information

• Transplantation: Translational and Transplant Pathology
  October 26, 2018 | Location: Exhibit Hall, San Diego Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Transplantation

• 1802 Transplantation: Clinical

Authors

• Lee, Yu ho, Kyung Hee University, Seoul, Korea (the Republic of)

Yu ho Lee,

• Jung-Woo, Seo, Kyung Hee University, Seoul, Korea (the Republic of)

Seo Jung-Woo,

• Jung, Su Woong, Kyung Hee University, Seoul, Korea (the Republic of)

Su Woong Jung,

• Kim, Yang gyun, Kyung Hee University, Seoul, Korea (the Republic of)

Yang gyun Kim,

• Moon, Ju young, Kyung Hee University, Seoul, Korea (the Republic of)

Ju young Moon,

• Kim, Jin sug, Kyung Hee University, Seoul, Korea (the Republic of)

Jin sug Kim,

• Jeong, Kyung-hwan, Kyung Hee University, Seoul, Korea (the Republic of)

Kyung-hwan Jeong,

• Chung, Byung ha, Seoul St. Mary's Hospital, Seoul, Korea (the Republic of)

Byung ha Chung,

• Lee, Sangho, Kyung Hee University, Seoul, Korea (the Republic of)

Sangho Lee,

Background

Acute rejection (AR) is the main obstacle to the graft survival after kidney transplantation. Urinary exosome is a promising source of biomarker for various kidney diseases, but few studies determined the clinical relevance of urinary exosomal microRNA in kidney transplant recipients. The purpose of this study was to investigate the profiles of urinary exosomal microRNAs to discover novel biomarkers of AR in patients who underwent kidney transplantation.

Methods

urinary exosomal microRNAs from 108 kidney transplant recipients were extracted. The candidate microRNAs for the diagnosis of AR were selected based on Nanostring analysis of urinary exosomal microRNAs, meta-analysis and the review of literature. The levels of candidate microRNAs were further confirmed by quantitative real-time polymerase chain reaction. The diagnostic value of final candidate microRNAs was determined in independent validation group.

Results

Nanostring analysis found that the expressions of 14 microRNAs were significantly altered in patients with AR compared to those with stable graft function. Meta-analysis and the review of literature revealed 10 and 7 additional candidate microRNAs of AR, respectively. Quantitative real-time polymerase chain reaction confirmed that a total of 7 microRNAs maintained their differential expressions, and 4 microRNAs (hsa-miR-21-5p, hsa-miR-30a-3p, hsa-miR-4488, and hsa-miR-4532) were chosen as final candidate microRNAs of AR by forward stepwise logistic regression model. The combinations of the levels of these 4 microRNAs effectively discriminated patients with AR from other patients in validation group, with an AUC value of 0.790.

Conclusion

This study demonstrated that the profiles of urinary exosomal microRNAs are altered in kidney transplant recipients having AR, and these differences could be potential non-invasive biomarkers for the diagnosis of AR.

Funding

• Government Support - Non-U.S.