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Abstract: FR-PO893

Proteomic Analysis of Perfusate from Machine Cold Perfusion of Transplant Kidneys: Comparison Between Circulatory and Neurological Death

Session Information

Category: Transplantation

  • 1802 Transplantation: Clinical

Authors

  • Angeletti, Andrea, University Hospital of Bologna, Bologna, Italy
  • Donadei, Chiara, University Hospital of Bologna , Bologna, Italy
  • Ravaioli, Matteo, University Hospital of Bologna , Bologna, Italy
  • De pace, Vanessa, University Hospital of Bologna, Bologna, Italy
  • Corradetti, Valeria, University Hospital of Bologna , Bologna, Italy
  • Comai, Giorgia, University Hospital of Bologna , Bologna, Italy
  • La Manna, Gaetano, University Hospital of Bologna , Bologna, Italy
Background

Renal donation after circulatory death (DCD) is associated with increased of delayed graft function (DGF) events shortly after transplant and with a decrease in renal function at 1 year compared to donation after neurological death (DND). Machine cold perfusion has been suggested to reduce incidence of DGF and improved long and short-term function in marginal kidneys and those obtained from DCD donors. We compared several early biomarkers of renal injury in perfusate of kidneys from DCD and DND.

Methods

Perfusates from DCD and DND were collected (n=25) at the beginning (T0) and at the end (T1) of perfusion and analyzed by Luminex technology for neutrophil gelatinase-associated lipocalin (NGAL), Cystatin C, Kidney Injury Molecule-1 (KIM-1), Monocyte chemoattractant protein-1 (MCP-1), and trefoil factor 3 (TFF3).

Results

Donor kidneys characteristics are resumed in Table. The highest levels of early biomarkers of renal tubular injury were seen for the DCD compared to DND kidneys for all markers except for MCP-1, the key chemokine that regulate migration and infiltration of monocytes/macrophages (Figure). No differences are reports for DGF of recipients.

Conclusion

Comparison of the perfusates from the different types of kidneys has allowed us to identify increased levels of biomarkers, representatives of increased acute tubular damage, that will be useful in future research into improving DCD kidneys outcome.

Table
 DND
n= 11
DCD
n= 14
p-value
Donor Age (yrs±SEM)59.9±1.461.9±2.3n.s.
Donor Gender (M:F)8:312:2n.s.
Donor baseline serum creatinine (mg/dl±SEM)0.7±0.070.9±0.13n.s.
Machine cold perfusion (min±SEM)184±29215±29n.s.
Machine cold perfusion flow (ml/min±SEM)47.4±5.359.9±7.9n.s.
Cold ischemic time (hours±SEM)14.3±1.19.8±0.80.013
Delayed graft function22n.s.

Funding

  • Clinical Revenue Support