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Abstract: SA-PO572

Hypoproteic Diet During Pregnancy and/or Lactation Differentially Affects the Response of the Offspring to an Episode of AKI

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • García-Ortuño, Luis Enrique, Molecular Physology Unit, Mexico City, Mexico
  • Barrera-Chimal, Jonatan, Molecular Physology Unit, Mexico City, DIstrito Federal, Mexico
  • Pérez-villalva, Rosalba, Molecular Physology Unit, Mexico City, Mexico
  • Ortega-Trejo, Juan Antonio, Molecular Physology Unit, Mexico City, Mexico
  • Lima Posada, Ixchel Quetzaliztli, Molecular Physology Unit, Mexico City, Mexico
  • Reyes castro, Luis Antonio, INCMNSZ, MEXICO, Mexico
  • Bobadilla, Norma, Molecular Physology Unit, Mexico City, Mexico
Background

Protein restriction during pregnancy induces morpho-functional alterations related to deficient nephrogenesis. We studied the renal functional and morphological significance of protein restriction during pregnancy (Preg), lactation (Lact) or both, in the adult stage of the offspring and their repercussion on the AKI severity.

Methods

Female rats were randomly assigned to the following groups: C = standard diet, (SD) during Preg and Lact; CR = SD during Preg and protein restriction diet (PR) during Lact; RC = PR during Preg and SD during Lact and RR = PR during Preg and Lact. Three months after birth, at least twelve male offspring of each group underwent randomly to bilateral renal ischemia for 45 min (IR) or sham surgery. Thus, eight groups were studied 24 h after reperfusion: C, C+IR, CR, CR+IR, RC, RC+IR, RR and RR+IR.

Results

The CR, RC, and RR groups exhibited a significant reduction by ≈ 15% in the nephron number that was associated with a reduction in renal blood flow (RBF). In spite of this, glomerular hypertrophy was observed together with a significant reduction in endothelin, angiotensinogen and their receptors, as well as catalase and GPx mRNA levels. In the next table appears the % change in renal function and vasoactive factors mRNA levels after the IR injury compared to their respective control group.

Conclusion

During basal conditions, the lesser nephrons observed in the offspring of protein-restricted mothers was associated with glomerular hypertrophy and reduced expression in vasoconstrictor factors. The ischemic insult in these groups induced a differential vasoconstrictor response that allowed a faster recovery in RBF, as compared to control group. These results suggest that CR, RC and RR groups respond better to an ischemic insult and most likely to the long-term consequences of AKI.

Percentage change in functional, vasoactive factor and antioxidant response against an AKI episode

Funding

  • Government Support - Non-U.S.