Abstract: FR-PO934
Comprehensive Gene Expression Analysis for Pax2 Related Genes with Existing FANTOM Database
Session Information
- Development, Stem Cells, Regenerative Medicine - II
October 26, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Development, Stem Cells, and Regenerative Medicine
- 501 Development, Stem Cells, and Regenerative Medicine: Basic
Authors
- Yamamura, Yuta, Kanazawa University, Kanazawa, Ishikawa, Japan
- Furuichi, Kengo, Kanazawa University, Kanazawa, Ishikawa, Japan
- Hara, Akinori, Kanazawa University, Kanazawa, Ishikawa, Japan
- Iwata, Yasunori, Kanazawa University, Kanazawa, Ishikawa, Japan
- Sakai, Norihiko, Kanazawa University, Kanazawa, Ishikawa, Japan
- Shimizu, Miho, Kanazawa University, Kanazawa, Ishikawa, Japan
- Wada, Takashi, Kanazawa University, Kanazawa, Ishikawa, Japan
Background
Pax2 is essential transcriptional factor for kidney development. Pax2 homo knockout mouse shows kidney agenesis and neonatal lethal. In human, PAX2 mutation is major causative genes for Renal coloboma syndrome (RCS), which is characterized by kidney hypoplasia or dysplasia and abnormality of the optic nerve. It is reported that Pax2 involved in ureteric bud branching via the regulation of Gdnf expression.
Pax2 is known to involve both gene expression and suppression through epigenetic mechanism, apart from the role of transcriptional factor.
In this study, using gene expression of mouse embryonic kidney from FANTOM database, comprehensive analysis for the genes which involved to Pax2 were evaluated. Furthermore, the role of PAX2 gene during human kidney development is not clear. Gene expression analysis in the kidney lineage cells differentiated from human induced pluripotent stem cells（iPSC）was performed.
Methods
To evaluate Pax2 related genes in mouse, we extracted gene expression data during kidney development (from embryonic day 14.5 to neonatal day 30). In the view of embryonic time course and organs, the correlation to Pax2 gene expression was evaluated. To evaluate PAX2 related gene in human, human iPSCwas differentiated into kidney lineage cells with reported methods (Taguchi, et al. Cell Stem Cells 2014). Some markers for kidney lineage cells were checked by immunocytochemistry and qPCR. Gene expressions of extracted candidate genes from mouse database were confirmed using the kidney lineage cells differentiated from human iPSC.
Results
About 180000 promoter expression data during mouse kidney development were extracted. The correlation to Pax2 promoter for time course and organswas evaluated. 3646 genes and 135 genes were extracted respectively. 18 genes, including some known essential genes for kidney development were overlapping. Human iPSC was differentiated to kidney lineage cells and checked PAX2 and other kidney lineage markers by immunocytochemistry and qPCR.A part of 18 genes were confirmed by qPCRin human differentiated kidney lineage cells.
Conclusion
Comprehensive analysis using FANTOM databaseis useful for identification of Pax2 related genes during kidney development.