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Abstract: FR-PO312

A Structural Equation Model on Regulatory Network of Phosphate Metabolism in Healthy Individuals

Session Information

Category: Fluid and Electrolytes

  • 902 Fluid and Electrolytes: Clinical

Authors

  • Ye, Guoxin, Division of Nephrology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
  • Wang, Mengjing, Huashan hospital, Fudan University, Shanghai, China
  • Zhang, Jiaying, Huashan hospital, Fudan University, Shanghai, China
  • Chen, Weisheng, Huashan Hospital, Shanghai, SHANGHAI, China
  • Chen, Jing, Huashan Hospital, Shanghai, SHANGHAI, China
Background

Basic and clinical studies reveal that the homeostasis of phosphate is maintained by a regulatory network. The factors in the network may change along with the dietary intake of phosphate. The aim of our study was to explore the variation of phosphatoninin with the change of diet.

Methods

A cross-over trial of 6 healthy volunteers was conducted. Each of them was given diet strictly: regular Pi diet (1500 mg/d), low Pi diet (LPD, 500 mg/d) and high Pi diet (HPD, 2300 mg/d), with a wash out period after diet intervention. The data were traditionally detected by ANOVA. In order to overcome limitations of the traditional correlation analysis, we used structural equation model (SEM) as a general framework for a new paradigm of analysis of multiple correlations.

Results

Pi was significantly higher in HPD. In contrast, Ca was lower in HPD. Meanwhile, LDP increased 1,25(OH)D and 25(OH)D. HPD leaded to increasing level of iPTH. With the gradual escalation of Pi intake, the FGF23 were upward trend. However, klotho didn't appear to be affected by dietary intervention by ANOVA. With the traditional comparison, it was difficult to reveal the overall phosphate network regulation. By SEM, we developed 3 networks for phosphate regulation. When healthy individual with regular Pi diet, Ca, Pi, iPTH, FGF23 and klotho formed a precise regulatory network. When HPD or LPD, vitamin D was closely associated with other phosphatonin, suggesting vitamin D played a prominent role in HPD or LPD. FGF23 and klotho had a close relationship in the network, especially in high Pi loads.

Conclusion

By SEM, the importance of klotho in phosphate regulation is revealed with the network diagrams. Meanwhile, the network demonstrates the importance of vitamin D in abnormal phosphate metabolism which need further investigation.

Funding

  • Government Support - Non-U.S.