ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on Twitter

Kidney Week

Abstract: PO0930

Comparison of the Effect of Calorie-Matched High Saturated Fat and High Unsaturated Fat Diets on Lysosomal Renal Injury in Non-Obese, Streptozotocin-Injected CD-1 Mice

Session Information

Category: Diabetic Kidney Disease

  • 601 Diabetic Kidney Disease: Basic

Authors

  • Yamaguchi, Tamio, Department of Clinical Nutrition, Faculty of Health Science, Suzuka University of Medical Science, Suzuka, Mie, Japan
  • Yoshimura, Aya, Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi, Japan
  • Kugita, Masanori, Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi, Japan
  • Kumamoto, Kanako, Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi, Japan
  • Shiogama, Kazuya, Division of Morphology and Cell Function, Faculty of Medical Technology, Fujita Health University, Toyoake, Aichi, Japan
  • Bae, Kyongtae Ty, Department of Radiology, University of Pittsburgh, Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Nagao, Shizuko, Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi, Japan
Background

Type 2 diabetes mellitus often causes renal injury characterized by autophagic vacuoles. Although many studies with comparisons of high fat versus a normal balanced diet have been reported in diabetic models, there are few studies that equalized calorie intake and body weights. We reported that a high fat diet induced renal injury with impaired lysosome-mediated autophagic degradation in streptozotocin (STZ) injected mice (ASN Kidney Week 2019). However, the effect of fat type, saturated- or unsaturated-fat, was not determined. In the current study, an AIN93M diet (CONT group) was compared to energy-matched lard derived high saturated fat (LARD group) and soybean oil derived high unsaturated fat (SOY OIL group) diets to compare their effects on biochemical markers and renal morphology with lysosome-associated membrane protein 1 (LAMP1) expression.

Methods

Male CD1 mice were randomly divided into three pair-fed groups with 380 kilocalorie/100g energy from 7 to 20 weeks of age. CONT group: AIN93M diet with 62% (w/w) cornstarch, 10% sucrose, 4% soybean oil and 5% cellulose; LARD group: Diet with 31% cornstarch, no sucrose, 22% lard oil and 28% cellulose; SOY OIL group: Diet identical to the LARD diet, except that soybean oil replaced lard oil. At 17 and 18 weeks of age, STZ (100mg/kg body wt) was injected. At 20 weeks of age, blood was taken for measurements of insulin, triglyceride, total cholesterol, ALT, AST, creatinine and SUN. Kidneys were prepared for H&E staining and immunohistochemical staining to detect LAMP1.

Results

Final body weight, total intake of water, food and energy were not different between all groups. No statistical differences in all blood biochemical markers were detected as well. In kidneys, the number of LAMP1-positive renal tubular lipid vacuoles was higher in LARD compared with SOY OIL and CONT groups, whereas no difference was shown between SOY OIL and CONT groups.

Conclusion

The results suggest that high intake of saturated-fat may aggravate lysosomal renal injury in a non-obese, streptozotocin-induced diabetes mellitus model.

Funding

  • Government Support - Non-U.S.