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Kidney Week

Abstract: PO0956

A Clinical and Pathological Study on Association of 4-Hydroxynonenal with Diabetic Kidney Disease

Session Information

Category: Diabetic Kidney Disease

  • 602 Diabetic Kidney Disease: Clinical

Authors

  • Peng, Zhimei, First Affliated Hospital of Jinan University, Guang Zhou City, Guang Dong Province, China
  • Liu, Fanna, First Affliated Hospital of Jinan University, Guang Zhou City, Guang Dong Province, China
Background

To explore the potential correlation between plasma 4-HNE level ,tubulointerstitial 4-HNE deposition and DKD renal tubular atrophy during disease progression.

Methods

59 patients with clinical diagnosis of DKD and 11 normal control were collected in the First Affiliated Hospital of Jinan University from Dec 2018 to Dec 2019. The 59 patients were divided into CKD Phase 1-3, 4-5 according to the estimate glomerular filtration rate(eGFR). Oxidative stress indicators 4-HNE, superoxide dismutase (SOD)were measured in DKD patients.34 cases of diabetic nephropathy(DN) diagnosed by biopsy in the hospital were divided into 3 groups(CKD 1-2,3,4-5).Biopsy cases were subjected to 4-HNE immunohistochemical staining. Univariate and multivariate logistic regression analysis was performed to identify independent risk factors of DKD incidence, and establishment of DKD eGFR multiple linear regression model was made.

Results

Compared with the normal group, oxidative stress index 4-HNE gradually increased in CKD phase 1-3, 4-5 groups, but SOD gradually decreased (P<0.05). Logistic regression analysis found that plasma 4-HNE is an independent risk factor for DKD. (P=0.008; OR=1.003,95%CI 1.001~1.006) Pearson correlation analysis showed that plasma 4-HNE levels were positively correlated with systolic blood pressure, mean arterial pressure, urea nitrogen, cystatin C and creatinine, and negatively correlated with hemoglobin and eGFR. The eGFR multiple linear regression model showed that eGFR is independently negatively correlated with tubulointerstitial 4-HNE expression(ß=-0.50,P<0.001), urea, history of hypertension, renal tubular atrophy, and independently positively correlated with hemoglobin(R2=0.84,P<0.001). Variance analysis revealed that there was a statistically significant difference between tubulointerstitial 4-HNE staining scores with the degree of renal tubular atrophy and interstitial infiltrates. (P< 0.05)

Conclusion

In the progression of DKD, tubular atrophy, anemia, hypertension were associated with oxidative stress, based on serum and staining of 4-HNE. Staining of 4-HNE can be used as a predictor of renal dysfunction, which may be related to tubular atrophy and interstitial infiltrates. 4-HNE is an independent risk factor for progression of DKD.

Funding

  • Clinical Revenue Support