Abstract: PO0162
Dietary Omega-3 Fatty Acids Alter the Lipid Mediator Profile and the Fatty Acid Composition of Membrane Phospholipids but Is Not Enough to Improve Renal Insufficiency
Session Information
- AKI Mechanisms - 1
October 22, 2020 | Location: On-Demand
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Shioda, Ryotaro, Department of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan
- Jo, Airi, Department of Biochemistry, Juntendo University Graduate School of Medicine, Tokyo, Japan
- Suzuki, Yusuke, Department of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan
Background
The efficacy of omega-3 fatty acids on ischemia-induced AKI has been reported, but the underlying mechanisms remain poorly understood. There have been no reports that demonstrated how dietary omega-3 fatty acids influenced the components of membrane phospholipids in the kidney. In this study, we focused on the effect of dietary omega-3 fatty acids on the membrane phospholipids components in the kidney, and examined the disease course of ischemia-induced AKI in the presence of the lipid mediator alterable by dietary omega-3 fatty acids.
Methods
Male 4-week-old wild-type Sprague-Dawley rats were fed for 2 months on AIN-93M, which contains 4% soy oil, or modified AIN-93M, which contains 4% perilla oil instead of soy oil. AKI was induced by unilateral ischemic reperfusion with right nephrectomy. Left renal ischemia was induced by using non-traumatic vascular clamps for 30 min. At 24h after reperfusion, left kidneys and serum were collected. The fatty acid composition of membrane phospholipids and lipid mediators were quantified by HPLC-tandem mass spectrometry (HPLC/MS/MS).
Results
In the kidney of omega-3 diet fed rats, the levels of arachidonic acid-derived proinflammatory lipid mediators, except for 5-HETE, were not reduced compared with omega-6 fed rats. Eicosapentaenoic acid (EPA) and EPA-derived lipid mediators were significantly increased in the kidney of omega-3 diet fed rats. Furthermore, membrane phospholipids which contained EPA and docosahexaenoic acid (DHA) were significantly increased in the kidney of omega-3 diet fed rats. However, there was no significant difference in serum creatinine, blood urea nitrogen or histological damage between omega-3 diet fed rats and omega-6 diet fed rats.
Conclusion
Dietary omega-3 fatty acids altered the lipid mediatorsthe and the fatty acid composition of membrane phospholipids, but was not enough to improve renal insufficiency or histological damage.