ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on Twitter

Kidney Week

Abstract: PO0162

Dietary Omega-3 Fatty Acids Alter the Lipid Mediator Profile and the Fatty Acid Composition of Membrane Phospholipids but Is Not Enough to Improve Renal Insufficiency

Session Information

  • AKI Mechanisms - 1
    October 22, 2020 | Location: On-Demand
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Shioda, Ryotaro, Department of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan
  • Jo, Airi, Department of Biochemistry, Juntendo University Graduate School of Medicine, Tokyo, Japan
  • Suzuki, Yusuke, Department of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan
Background

The efficacy of omega-3 fatty acids on ischemia-induced AKI has been reported, but the underlying mechanisms remain poorly understood. There have been no reports that demonstrated how dietary omega-3 fatty acids influenced the components of membrane phospholipids in the kidney. In this study, we focused on the effect of dietary omega-3 fatty acids on the membrane phospholipids components in the kidney, and examined the disease course of ischemia-induced AKI in the presence of the lipid mediator alterable by dietary omega-3 fatty acids.

Methods

Male 4-week-old wild-type Sprague-Dawley rats were fed for 2 months on AIN-93M, which contains 4% soy oil, or modified AIN-93M, which contains 4% perilla oil instead of soy oil. AKI was induced by unilateral ischemic reperfusion with right nephrectomy. Left renal ischemia was induced by using non-traumatic vascular clamps for 30 min. At 24h after reperfusion, left kidneys and serum were collected. The fatty acid composition of membrane phospholipids and lipid mediators were quantified by HPLC-tandem mass spectrometry (HPLC/MS/MS).

Results

In the kidney of omega-3 diet fed rats, the levels of arachidonic acid-derived proinflammatory lipid mediators, except for 5-HETE, were not reduced compared with omega-6 fed rats. Eicosapentaenoic acid (EPA) and EPA-derived lipid mediators were significantly increased in the kidney of omega-3 diet fed rats. Furthermore, membrane phospholipids which contained EPA and docosahexaenoic acid (DHA) were significantly increased in the kidney of omega-3 diet fed rats. However, there was no significant difference in serum creatinine, blood urea nitrogen or histological damage between omega-3 diet fed rats and omega-6 diet fed rats.

Conclusion

Dietary omega-3 fatty acids altered the lipid mediatorsthe and the fatty acid composition of membrane phospholipids, but was not enough to improve renal insufficiency or histological damage.