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Abstract: PO2274

Markers of Trans IL-6 Signaling Are Not Differentially Induced During Febrile and Afebrile Urinary Tract Infection

Session Information

Category: Pediatric Nephrology

  • 1700 Pediatric Nephrology

Authors

  • Gupta, Sudipti, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Lautzenhiser, Sara E., Nationwide Children's Hospital, Columbus, Ohio, United States
  • Spencer, John David, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Becknell, Brian, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Ching, Christina B., Nationwide Children's Hospital, Columbus, Ohio, United States
Background

Febrile urinary tract infections (UTIs) are generally thought to be evidence of tissue inflammation such as pyelonephritis as compared to afebrile UTIs which are thought to be more localized to the bladder. As such, generally the concern for renal damage is more in those with febrile UTI and thought to be a result of the inflammatory response generated. IL-6 is a known mediator of inflammation, particularly through its trans signaling pathway. We hypothesized there would be differences in markers of trans IL-6 signaling in the urine of children with febrile compared to afebrile UTI.

Methods

Pediatric patients with signs of active UTI were consented/assented for participation. Urine was collected at time of evaluation for active UTI in the urology or nephrology office or at hospitalization and were divided into those with a fever (≥38°C) compared to those without (<38°C). Patients were included for analysis if they had a positive urine culture (>50K CFU of a uropathogen) and for those without a fever if they had urinary symptoms such as dysuria, urgency, frequency, and/or new or worse urinary incontinence. Those with fever were termed a febrile UTI and those without a fever were termed afebrile UTI. Enzyme-linked immunosorbent assays were performed on samples for markers of trans IL-6 signaling: IL-6, soluble (s)IL-6 receptor (R), and soluble (s)gp130. Results were normalized to urine creatinine. Results were analyzed by Mann-Whitney U. A p-value of <0.05 was considered significant.

Results

17 patients with febrile UTI and 23 patients with afebrile UTI were included. Two of the patients with febrile UTI were male while all of the afebrile UTI patients were female (p=0.1258). The groups did not differ significantly based on age (p=0.6218). While we found that those with a febrile UTI had a higher IL-6 in their urine at the time of collection (p=0.0479), there was no significant difference in expression of sIL-6R or sgp130. Ratios of these markers also were not significantly different.

Conclusion

Markers of trans IL-6 signaling, either as absolute values or ratios, are not different between individuals at the time of febrile or afebrile UTI.

Funding

  • NIDDK Support