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Abstract: TH-OR15

A Single-Cell Transcriptome Atlas of Adult Parathyroids in Secondary Parathyroidism

Session Information

Category: Bone and Mineral Metabolism

  • 401 Bone and Mineral Metabolism: Basic

Authors

  • Li, Shensen, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
  • Li, Wenwen, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
  • Liu, Qingqing, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
  • Shen, Haiyan, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
  • Deng, Bingquan, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
  • Cao, Changchun, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
Background

Secondary hyperparathyroidism (SHPT) is one of the common complications in patients with chronic kidney disease. The development of SHPT is accompanied by the change of cell composition, while the exact cell type changes and mechanism are yet to be defined. Therefore, single cell sequencing was conducted to analysis the cell composition of parathyroid gland.

Methods

In current study, parathyroids from 3 SHPT patients were digested to obtain single cell suspension. A total of 21519 cells were obtained and the mRNA expression profiles were analyzed by single cell sequencing and bioinformatics. Furthermore, the development separation track of cell subpopulations was constructed by pseudotime analysis (Figure 1A).

Results

There may be 21 cell subpopulations in parathyroid, among which 6 subpopulations (clusters 0, 1, 2, 5, 11, 17) are high function subpopulations of parathyroid, which were indicated by high expression of gcm2 (glial cells missing homolog 2), PTH (parathyroid hormone), CaSR (calcium sensing receptor) and KL (Klotho) genes (Figure 1B-D). The results of pseudotime analysis in the 6 high function subpopulations show that cluster 0 is at the beginning of the main group separation track, cluster 1, 2, 5 are in the middle, while cluster11 and 17 are at the end (Figure 1E). Multiple genes may play major role in the differentiation of cluster 0, including tspan1, park7, atp6v0b, rpl11, rps8 and etc (Figure 1F).

Conclusion

There are 6 subpopulations out of total 21 cell subpopulations of parathyroid cells with higher parathyroid hormone secretion and regulation function in SHPT patients. Among which, cluster 0 may be the initiation differentiation cell of high functional cells, because it may be a subpopulation with high proliferation and differentiation potential.

Identification and analysis of subpopulations of uremic hyperparathyroidism glands.

Funding

  • Government Support - Non-U.S.