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Kidney Week

Abstract: PO0308

Nitric Oxide (NO) Based Urinary Catheter Balloon Inflation Solution to Prevent Urinary Tract Infection

Session Information

  • Bioengineering
    October 22, 2020 | Location: On-Demand
    Abstract Time: 10:00 AM - 12:00 PM

Category: Bioengineering

  • 300 Bioengineering

Authors

  • Yevzlin, Alexander S., University of Michigan, Ann Arbor, Michigan, United States
  • Ramani, Karthik, University of Michigan, Ann Arbor, Michigan, United States
  • Suliman, Faroug, University of Michigan, Ann Arbor, Michigan, United States
Background

UTI is the most common hospital acquired infection with indwelling catheters being a major risk factor and are difficult to treat due to the formation of bacterial biofilms which are resistant to systemic antibiotics. NO is an endogenously formed gas molecule known to play a key role in preventing infection by dispersing biofilm formed by a variety of bacterial strains. In this abstract, we describe the effectiveness of a novel urinary catheter balloon inflation fluid to effectively reduce catheter associated urinary tract infections (CAUTI) by providing up to 7 days of bactericidal effect via NO release.

Methods

Our innovative approach to prevent CAUTI involves employing a balloon inflation fluid using novel NO secreting materials based on using S-nitrosothiol type NO donors like S-nitrosoglutathione (GSNO) within the balloon of urinary catheters that slowly releases NO over a period of up to one week. The advantages of the use of NO in CAUTI prevention is its short half-life with a very low steady-state level immediately adjacent to the surface of the device required to achieve the desired anti-microbial effect with no risk of systemic effects when using NO secreting materials with fluxes that are near physiological levels.

Results

We performed in vitro studies using a Foley catheter placed in a long-necked flask with a shape resembling the urinary bladder and the urethra. (Fig 1) The Foley catheter retention balloon was filled with GSNO solution and the balloon was used to seal the neck of the flask. Then the flask was filled with synthetic urine inoculated with E. coli and incubated for seven days at 37 0C with horizontal shaking at 80 rpm. The results showed a 7-log reduction in planktonic bacterial growth (Fig 2) and a 3-log reduction in biofilm (Fig 3) of the GSNO Foley balloon solution compared to the control.

Conclusion

These data suggest that NO-based urinary catheter balloon fluid results in significant anti-microbial effects in our in vitro model of CAUTI.