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Abstract: PO1719

In Silico Prediction of Potential New Biomarkers of IgA Nephropathy Interstitial Lesion

Session Information

Category: Glomerular Diseases

  • 1201 Glomerular Diseases: Fibrosis and Extracellular Matrix

Authors

  • Zhu, Fengge, Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, Beijing, China
  • Cai, Guangyan, Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, Beijing, China
  • Ke, Yujing, Guangdong Pharmaceutical University, Guangzhou, China
  • Chen, Xiangmei, Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, Beijing, China
Background

IgA nephropathy remains one of the major causes of end stage renal diseases globally. Interstitial lesion in IgA nephropathy is correlated with unfavorable prognosis. This study aims to find new potential biomarkers in IgA nephropathy patients with interstitial lesion based on an in silico method.

Methods

Proteomics matrix data from IgA nephropathy patients are obtained from a local renal biopsy patient cohort. Discovery is determined using the Two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 1%. Transcriptomic data from peripheral mononuclear blood cell of IgA nephropathy patients are obtained from GEO (GSE73953). Detection of transcriptomic difference genes are made with limma method in GEO2R.

Results

Multiple t test indicates 887 differentially expressed genes between IgA nephropathy interstitial lesion (T1 or T2) and control renal tissues. KEGG pathway annotation reveals that cytochrome p450 related drug metabolism pathway and oxidative phosphorylation pathway are significantly clustered in IgA nephropathy patients with interstitial lesion. No herbal medicine or drug use (apart from ACEI or ARB) were recorded. Differential gene analysis reveals a total of 250 genes with positive discoveries in peripheral mononuclear blood cells of IgA nephropathy compared with membranous nephropathy (GSE73953). Further screening of overlapping genes demonstrates that ABCD3, CLPTM1, FMO4, RARS2, SFXN2 are the most significantly enriched proteins in IgA patients with interstitial lesion.

Conclusion

Preliminary results from this in silico study of proteomics and transcriptomics data in IgA nephropathy patients using a T score specific and overlapping screening approach provide a new possibility of noninvasive detection of interstitial lesion in IgA nephropathy patients.