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Abstract: PO2273

Trans IL-6 Signaling Does Not Distinguish Between Pediatric Patients with and Without Scarring After Febrile Urinary Tract Infection

Session Information

Category: Pediatric Nephrology

  • 1700 Pediatric Nephrology

Authors

  • Gupta, Sudipti, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Lautzenhiser, Sara E., Nationwide Children's Hospital, Columbus, Ohio, United States
  • Spencer, John David, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Becknell, Brian, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Ching, Christina B., Nationwide Children's Hospital, Columbus, Ohio, United States
Background

The inflammatory response generated in response to infection is believed to be largely responsible for the development of renal scarring after UTI. IL-6 is a cytokine known to be induced during UTI with a pro-inflammatory pathway, known as trans signaling. We hypothesized there would be differences in markers of trans IL-6 signaling between patients with a history of febrile urinary tract infection (UTI) who had subsequent renal scarring as compared to those with a history of febrile UTI who did not develop renal scarring.

Methods

Urine samples were collected on consenting/assenting pediatric patients with a history of febrile (≥38°C) UTI (urine culture >50K uropathogen) with documented presence or absence of renal scarring on imaging. Patients were not actively infected at time of sample collection. Enzyme-linked immunosorbent assays were performed on samples for markers of trans IL-6 signaling: IL-6, soluble (s)IL-6 receptor (R), and soluble (s)gp130, a buffer in trans IL-6 signaling. Values were normalized to urine creatinine. Results were analyzed by t-test or Mann-Whitney U. Spearman rank correlation was used. A p-value of <0.05 was considered significant.

Results

50 urines from patients with a history of febrile UTI were collected: 23 with and 27 without scarring. The groups were not significantly different in age or gender. Urine IL-6, sIL-6R, or sgp130 were not significantly different between those with and without scarring. While IL-6 values significantly correlated with sgp130 values (p=0.0004) in those without renal scarring, the values did not correlate in those with scarring (p>0.05). Ratios of IL-6:sgp130 and sIL-6R:sgp130 were not different between groups.

Conclusion

There is a suggestion of altered buffering of trans IL-6 signaling in those with renal scarring compared to those without due to a lack of correlation of sgp130 with IL-6 in those with scarring. The absolute values and ratios of these markers of trans IL-6 signaling, however, are not significantly different between individuals with a history of febrile UTI with and without renal scarring in the non-acute setting.

Funding

  • NIDDK Support