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Abstract: PO1734

The Compositional and Functional Changes of Gut Microbiota in Crescentic Glomerulonephritis

Session Information

Category: Glomerular Diseases

  • 1202 Glomerular Diseases: Immunology and Inflammation

Authors

  • Yin, Huanhuan, Peking Union Medical College Hospital, Beijing, Beijing, China
  • Shi, Xiaoxiao, Peking Union Medical College Hospital, Beijing, Beijing, China
  • Xia, Peng, Peking Union Medical College Hospital, Beijing, Beijing, China
  • Chen, Limeng, Peking Union Medical College Hospital, Beijing, Beijing, China

Group or Team Name

  • Department of Nephrology, Chinese Academy of Medical Sciences & Peking Union Medical College Hospital, Beijing, China
Background

Crescent glomerulonephritis(CreGN) is the most dangerous glomerulonephritis, but the specific mechanism remains unclear.Recent evidences suggested that gut microbiota was associated with kidney injury.This study aimed to observe the role of gut microbiota in CreGN.

Methods

A comparative analysis of the gut microbiota was performed in 10 CreGN patients and 10 matched healthy controls by 16S rDNA amplicon sequencing, and half of each group were also analyzed by metagenomics for further function analysis.

Results

The mean age of the 10 CreGN patients was 54.8±17.1 years, with 70.0% were female.The median eGFR was 5.4(interquartile range:3.6-9.5)ml/min/1.73m2.Compared to controls,CreGNs showed a significant lower richness(683.2±113.4 vs.800.1±73.5, P= 0.015) despite a similar level of Shannon index(diversity)(5.4±0.6 vs.5.6±0.5,P=0.579).PCoA showed a different pattern of clustering in CreGN(P=0.036, Fig.1a).There were 66 differentially enriched bacteria genera,with a significant(q<0.05) lower relative abundance of Agathobacter, Prevotellaceae, Faecalibacterium, Dialister, Megamonas, and Alloprevotella genera, and a higher relative abundance of Faecalitalea, Anaerostipes, Paraprevotella, and Streptococcus genera(top 10)in CreGN.All the genera above showed significant correlations with renal injury indicators.For example,Agathobacter was significantly correlated with creatinine(r =−0.77),24h urine protein(r =−0.86),and eGFR(r=0.73)(Fig.1b).Besides,Agathobacter, Prevotellaceae, Faecalibacterium, and Dialister were also confirmed as significant biomarkers by LEfSe(Fig.1c).Metagenomics showed 21 significant differentially enriched functional categories,with a decreased proportion of genes involved in“digestive system"(q=0.022) and an increased risk of“infectious diseases: bacterial"(q= 0.011) in CreGN.

Conclusion

Gut microbiota in CreGN differed at compositional and functional levels compared with healthy controls, and correlated well with renal injuries.

Figure 1. Altered gut microbiota in CreGN. a)PCoA. b)LEfSe. c)Spearman correlation. A1:CreGN,A2:Control.

Funding

  • Government Support - Non-U.S.