ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on Twitter

Kidney Week

Abstract: PO0843

Oxidative Stress, the Final Common Pathway in Lung-Kidney Pathophysiological Cross-Talk in an Experimental Model of COVID-19: Clinical Implications

Session Information

Category: Coronavirus (COVID-19)

  • 000 Coronavirus (COVID-19)

Author

  • Farahmand, Firoozeh, Saint Louis University, Saint Louis, Missouri, United States
Background

AKI occurs frequently in patients with COVID-19 disease early in the course, in temporal association with respiratory failure and is associated with a poor prognosis. AKI is primarily seen in Covid-19 patients with respiratory failure, with 90% of patients on mechanical ventilation developing AKI compared to 22% of non-ventilated patients. To develop experimental models investigating pathophysiological mechanism of Lung- kidney inetarctions is an essential part of understanding the mechanisms of organs cross talk, i.e., the complex biological communication and feedback between distant organs mediated via cellular and molecular pathways.

Methods

In a novel experimental model similar to human COVID-19, ARDS followed by AKI developed by single injection of a Toxoid.(TOX). Two days post injection lungs and kidney were analyzed for antioxidant enzyme activities including superoxide dismutase, glutathione peroxidase, catalase and oxidative stress. Lungs wet/dry weight ratios were measured to evaluate edema. After sacrificing the animals, kidney and the lung were removed for histology.

Results

At 2 days post-TOX injection there was acute lung injury with cytotoxin influx, lung edema, neutrophil infiltration, hypoxemia and pulmonary artery thrombosis. In the kidney there was acute tubular necrosis with inflammatory infiltration. Oxidative stress was increased in the lung and the kidney. Antioxidant enzymes activities of SOD and GSHPx were decreased in the lung and the kidney.

Conclusion

In this experimental model mimicking COVID-19 organ failure , AKI and ARDS in rats correlates with a decrease in antioxidant and increase in oxidative stress in the lung & the kidney. This suggest the role of antioxidant as the potential adjunct therapeutic agents in COVID-19 related organ failure.