Extraction of <i>Escherichia coli</i> in Urine by New Static Electricity Technique In Vitro
October 22, 2020 | 10:00 AM - 12:00 PM
Extraction of Escherichia coli in Urine by New Static Electricity Technique In Vitro
October 22, 2020 | Location: On-Demand
Abstract Time: 10:00 AM - 12:00 PM
- 300 Bioengineering
- Yamamoto, Tokunori, Dept of nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan
- Maruyama, Shoichi, Dept of nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan
- Morita, Satoshi, AFI Corporation, Kyoto, Japan
- Wakizaka, Yoshikazu, AFI Corporation, Kyoto, Japan
The treatment results for sepsis are poor due to infectious diseases, and the mortality rate is over 25%. It is clinically significant that the detection identifies patient specimen primary causative organisms as promptly as possible to give appropriate antimicrobial treatment, and to save patients with serious infectious disease. However, it usually takes 2-3 days from the submission of the sample to the identification of the primary causative organism.We newly developed the device (PixeeMoTM approved by AOAC® Performance TestedSM Certificate No. 012002 in January 14, 2020.) with quick extraction of bacteria in drinking water by static electricity technique. In this report, we evaluated the ability of PixeeMoTM to extract E. coli from urine.
Samples were prepared by adding Ecoli to artificial urine. 27 mL of a dedicated buffer was added to 3 mL of the sample, and after centrifugation (8000 xg, 20 min), 27 mL of the supernatant was removed. This operation was performed 3 times. After the preparation, E. coli was extracted from each sample using PixeeMoTM less than 0.5 hour. The number of bacteria in the sample prepared on the standard agar medium was measured by colony count.
The table shows results of the experiments. The components of artificial urine and E. coli were separable and the extraction results by PixeeMo were consistent with the culture method. It was also suggested that the detection limit concentration is 10<font size="1"> </font>cells / mL.(Table)
The new technique could detect clinical pathological conc. of E. coli in short time less than 2.0 hour. Extremely useful possibility is suggested as the new measurement technology of sepsis to reflect a diagnosis and evaluation of treatment, curative effect, very quickly.