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Abstract: PO2227

Single-Cell Transcriptomics of the Peripheral Blood Revealed Inflammation and Infection Were Associated with IgAN

Session Information

Category: Pathology and Lab Medicine

  • 1601 Pathology and Lab Medicine: Basic

Authors

  • Wu, Changwei, Renal Department and Nephrology Institute, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China
  • Li, Guisen, Renal Department and Nephrology Institute, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China
  • Wang, Li, Renal Department and Nephrology Institute, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China
Background

Immunoglobulin A nephropathy (IgAN) is the most common primary glomerular disease worldwide. Due to the diverse clinical manifestations and the complex pathological features, IgAN patients have different response to therapy, and the optimal treatment for IgAN remains controversial. Our study investigated the pathogenesis of IgAN by single-cell transcriptome sequencing.

Methods

Single-cell transcriptome sequencing was performed on peripheral blood monocytes derived from 3 IgAN patients and 1 healthy control, and differential gene expression profiles of peripheral blood single-cell were established. Functional analysis was performed to explore the pathogenesis of IgAN. Meanwhile, RT-PCR was used to validate the differential expression of mRNA and miRNA.

Results

According to quality control and cell selection, we characterized 21739 cells using unbiased single-cell RNA sequencing. 3 IgAN patients included 7847, 5389 and 6609 cells, respectively, and the helthy control included 1894 cells. We used unsupervised clustering to cluster cell clusters. In addition, cells were divided into 14 cell groups, including B lymphocytes and T lymphocytes, based on cell markers (Figure 1). Functional analysis revealed that differential genes were extensively enriched in inflammation / infection-related pathways in each cell type (Figure 2), and the EBV infection pathway focused on antigen presentation (Figure 3). RT-PCR of B lymphocytes demonstrated that SPI1, MXD1 and S100A9 mRNA levels were higher in IgAN patients than controls (Figure 4), which showed the reults of single-cell transcriptome sequencing was avalible.

Conclusion

Differential gene expression profiles of IgAN in peripheral blood single cells were successfully established, and it demonstrated that the inflammation/infection pathway was associated with IgAN.