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Kidney Week

Abstract: PO0911

Investigations on Urinary Exosomal MiRNA Biomarkers That Reflect Pathologic Features of Patients with Diabetic Kidney Diseases

Session Information

Category: Diabetic Kidney Disease

  • 601 Diabetic Kidney Disease: Basic

Authors

  • Jung-Woo, Seo, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Korea (the Republic of)
  • Lee, Yu ho, Division of Nephrology, Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea (the Republic of)
  • Kim, Miji, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Korea (the Republic of)
  • Kim, Yang gyun, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Korea (the Republic of)
  • Lee, Sangho, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Korea (the Republic of)
  • Kim, Jin sug, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Medical Center, Seoul, Korea (the Republic of)
  • Hwang, Hyeon Seok, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Medical Center, Seoul, Korea (the Republic of)
  • Jeong, Kyung hwan, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Medical Center, Seoul, Korea (the Republic of)
  • Jeong, Hye yun, Division of Nephrology, Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea (the Republic of)
  • Lee, So-young, Division of Nephrology, Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea (the Republic of)
  • Yang, Dong Ho, Division of Nephrology, Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea (the Republic of)
  • Moon, Ju young, Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Korea (the Republic of)
Background

Diabetic kidney disease (DKD) is a leading cause of end-stage renal disease worldwide. Although histological severity of DKD is a well-established predictor of adverse renal outcomes, investigations on the identification of non-invasive biomarkers that can reflect intrarenal status are scarce. The aim of this study was to discover urinary exosomal miRNA biomarkers of DKD and to examine their associations with the degree of various pathologic injury scores.

Methods

We collected and analyzed urinary samples obtained from 95 patients with biopsy-proven DKD and 32 healthy controls. The candidate microRNAs of DKD were selected based on the analysis of GEO dataset (GPL22945 and GPL24120) and public microRNA databases (miRTarBase, TargetScan, microRNA). Urinary exosomes were extracted by column-based isolation kit, and the levels of selected candidate microRNAs were measured by quantitative real-time polymerase chain reaction.

Results

Mean estimated glomerular filtration rate and urinary protein-to-creatinine ratio of enrolled DKD patients were 46.0 mL/min/1.73 m2 and 6.8 g/g creatinine, respectively. Upon the analysis of public dataset, we identified 11 candidate microRNAs for DKD, whose expression in urinary exosomes were all significantly higher in patients with DKD compared to controls. In particular, urinary exosomal miR-30a-5p and miR-335-3p levels showed positive correlation with the degree of interstitial inflammation and arterial hyalinosis, respectively. There was no significant association between the remaining microRNAs and the degree of glomerular injury, tubulointerstitial fibrosis, or arteriosclerosis. Finally, we found significant correlation between urinary protein-to-creatinine ratio and the levels of urinary exosomal miR-98-5p.

Conclusion

Urinary exosomal microRNAs could reflect the degree of intrarenal pathologic status in patients with diabetic kidney disease.