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Kidney Week

Abstract: PO2307

Association of XOR Activity and NLRP3 Inflammasome Among CKD Patients

Session Information

Category: CKD (Non-Dialysis)

  • 2101 CKD (Non-Dialysis): Epidemiology, Risk Factors, and Prevention

Author

  • Yiyuan, Zhang, Xuzhou Medical University, Xuzhou, Jiangsu, China
Background

Previous studies have shown few result on the relationship between XOR activity and NLRP3 inflammatory in non-hemodialysis patients.

Methods

CKD patients in nephrology department with normal uric acid were recruited.General clinical data, blood biochemistry and other data were collected.XOR activity was detected by fluorescence colorimetry,XO activity was detected by double antibody sandwich ELISA, and NLRP3 Inflammasome were detected by competitive binding method.After correlation analysis, multiple (stepwise) regression analysis was performed to explore the correlation between XOR and NLRP3 inflammasome and the relationship with clinical data of patients.

Results

The correlation analysis of XOR activity, XO activity,XO/XOR ratio and NLRP3 Inflammasome with biochemical indicators showed that XOR activity was positively correlated with eGFR,DBP,FBG,and alanine transferase, and was negatively correlated with age, serum total protein,serum creatinine, and serum chloride concentration; Log(XO) was positively correlated with eGFR and FBG, and negatively correlated with serum chloride concentration;The ratio of XO/XOR was positively correlated with total protein, age and UA, and negatively correlated with eGFR, XOR and alanine transferase;NLRP3 inflammasome were positively correlated with XOR, Log(XO) and serum sodium concentration, and negatively correlated with sex;Multiple linear (stepwise) regression results showed that eGFR, FBG and DBP were independent influencing factors of XOR;eGFR,FBG,UA and total cholesterol were independent influencing factors of Log(XO); Serum creatinine,serum sodium concentration and XOR activity were independent influencing factors of NLRP3 Inflammasome, and there was no collinearity in statistical analysis. According to the value of eGFR, the patients were divided into two groups.The XOR activity,Log (XO),XO/XOR ratio,serum creatinine, urea nitrogen,UA, K, serum chloride concentration were compared between the two groups, and the differences were statistically significant.

Conclusion

In CKD patients, elevated fasting blood glucose and diastolic blood pressure are independent risk factors for XOR activity, while elevated XOR activity is an independent risk factor for NLRP3 Inflammasome. Therefore, controlling FBG and DBP in CKD patients has certain clinical reference significance for reducing XOR activity and further reducing NLRP3 Inflammasome.