Kidney Week

Abstract: PO0420

Air Pollution Aggravates Ischemia-Reperfusion-Induced AKI in Mice

Session Information

• AKI: Repair and Progression
  November 04, 2021 | Location: On-Demand, Virtual Only
  Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

• 103 AKI: Mechanisms

Authors

• Parra, Antonio Carlos, Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Antonio Carlos Parra

• Tammaro, Alessandra, Department of Pathology, Amsterdam UMC, Amesterdam Infection & Immunity Institute, University of Amsterdam, Amsterdam, Netherlands

Alessandra Tammaro,

• Graner, Mariana, Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Mariana Graner,

• Umesaki Itto, Lucas Yuji, Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Lucas Yuji Umesaki Itto,

• Kers, Jesper, Department of Pathology, Amsterdam UMC, Amesterdam Infection & Immunity Institute, University of Amsterdam, Amsterdam, Netherlands

Jesper Kers,

• Roelofs, Joris, Department of Pathology, Amsterdam UMC, Amesterdam Infection & Immunity Institute, University of Amsterdam, Amsterdam, Netherlands

Joris Roelofs,

• Veras, Mariana, Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Mariana Veras,

• Rodrigues, Camila Eleuterio, Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Camila Eleuterio Rodrigues,

• Florquin, Sandrine, Department of Pathology, Amsterdam UMC, Amesterdam Infection & Immunity Institute, University of Amsterdam, Amsterdam, Netherlands

Sandrine Florquin,

• Sanches, Talita R., Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Talita R. Sanches,

• Andrade, Lucia, Nephrology, University of Sao Paulo School of Medicine, Sao Paulo, Sao Paulo, Brazil

Lucia Andrade,

Background

The biggest city in Latin America is São Paulo (SP), where disorganized urbanization has had a negative impact on air quality and vehicle emissions are the main source of fine particulate matter (PM_2.5). Epidemiological studies have linked PM_2.5 exposure to an increased risk of CKD. The mechanisms mediating the adverse health effects of PM_2.5 include epigenetic changes, oxidative stress and inflammation. The role of PM_2.5 in AKI has yet to be described. We hypothesized that PM_2.5 exposure would aggravate renal ischemia/reperfusion (I/R) injury in mice.

Methods

In temperature-/humidity-controlled chambers within an ambient particle concentrator, animals were exposed to a concentrated PM_2.5 stream (PM_2.5) or to high-efficiency particulate air-filtered clean air (CA). Mass concentrations of PM were measured with an airborne particulate monitor, and the target dose was 600 µg m⁻³/day (equivalent of the daily exposure in SP). After 12 weeks, some PM_2.5 and CA mice underwent bilateral 30-min clamping of the kidney hila and subsequent reperfusion. All studies were performed 48 h after I/R. Groups: CA, PM_2.5, CA+I/R and PM_2.5+I/R. Data are mean±SEM.

Results

Renal TLR4 protein expression was higher in CA+I/R and PM_2.5+I/R than in CA and PM_2.5 (128±2.1 and 146±2.0 vs. 97.5±2.1 and 98.0±0.9%; P<0.05), also being much higher in PM_2.5+I/R than in CA+I/R (P<0.05). Manganese superoxide dismutase levels were higher in PM_2.5+I/R than in CA+I/R, AF and PM_2.5 (146±12 vs. 99±3.6, 102±3.9 and 96±2.8; P<0.05).

Conclusion

PM_2.5 aggravates I/R-induced AKI by decreasing renal Klotho protein, leading to increased renal TLR4 expression and inflammatory cell infiltration. (FAPESP, NWO)

Biochemistry and histology

*Immunohistochemical analysis; ^aP<0.05 vs. CA, PM2.5 and CA+I/R; ^bP<0.05 vs. CA and PM2.5.

Funding

• Government Support – Non-U.S.